CoraLite®594-conjugated Annexin A2 Monoclonal antibody

• Featured Product
• KD/KO Validated

Annexin A2 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1C1E12

Cat no : CL594-66035

Synonyms

Annexin 2, annexin A2, Annexin II, ANX2, ANX2L4, ANXA2, CAL1H, Calpactin 1 heavy chain, Calpactin I heavy chain, Chromobindin 8, LIP2, Lipocortin II, LPC2, LPC2D, P36, PAP IV, Protein I

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Validation Data Gallery

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  IF Staining of T-47D using CL594-66035

  Immunofluorescent analysis of (-20°C Ethanol) fixed T-47D cells using CoraLite®594-conjugated Annexin A2 antibody (CL594-66035, Clone: 1C1E12 ) at dilution of 1:600.

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Tested Applications

• Positive IF/ICC detected in: T-47D cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL594-66035 is Annexin A2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Annexin A2 fusion protein Ag16654
• Full Name: annexin A2
• Calculated Molecular Weight: 339 aa, 39 kDa
• GenBank Accession Number: BC016774
• Gene Symbol: Annexin A2
• Gene ID (NCBI): 302
• RRID: AB_2919940
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage.

Background Information

Annexin A2 (ANXA2), also named Annexin-2 or Lipocortin II, is a Ca2+ binding protein that is up-regulated in virally transformed cell lines and human tumors. This calcium-regulated membrane-binding protein, whose affinity for calcium is greatly enhanced by anionic phospholipids, may cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis. Heat-stress response may also involve Annexin-2.