Afp Recombinant antibody

Afp Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240232C7

Cat no : 83367-2-RR

Synonyms

Alpha-fetoprotein, Alpha-fetoglobulin, Alpha-1-fetoprotein, alpha fetoprotein, 240232C7

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Validation Data Gallery

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  WB analysis of mouse placenta using 83367-2-RR

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 83367-2-RR (Afp antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse placenta tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83367-2-RR is Afp Fusion Protein expressed in E. coli.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: alpha fetoprotein
• Calculated Molecular Weight: 67KD
• Observed Molecular Weight: 67-72 kDa
• GenBank Accession Number: NM_007423.4
• Gene Symbol: Afp
• Gene ID (NCBI): 11576
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Alpha-fetoprotein (AFP) is one of the earliest proteins to be synthesised by the embryonic liver. The synthesis of AFP decreases dramatically after birth and only trace amounts are expressed in the adult liver. High serum AFP concentrations are also found in connection with primary liver cancer and teratocarcinoma.