Afp Polyclonal antibody

Afp Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 31256-1-AP

Synonyms

Alpha-fetoprotein, Alpha-fetoglobulin, Alpha-1-fetoprotein, alpha fetoprotein

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Validation Data Gallery

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  WB analysis using 31256-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31256-1-AP (Afp antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 31256-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31256-1-AP (Afp antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse placenta tissue, HepG2 cells, human placenta tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31256-1-AP targets Afp in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: alpha fetoprotein
• Calculated Molecular Weight: 67KD
• Observed Molecular Weight: 68-72 kDa
• GenBank Accession Number: NM_007423.4
• Gene Symbol: Afp
• Gene ID (NCBI): 11576
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Alpha-fetoprotein (AFP) is one of the earliest proteins to be synthesised by the embryonic liver. The synthesis of AFP decreases dramatically after birth and only trace amounts are expressed in the adult liver. High serum AFP concentrations are also found in connection with primary liver cancer and teratocarcinoma.