Mouse Adiponectin Recombinant antibody, PBS Only (Detector)

Adiponectin Uni-rAb^TM Recombinant Antibody for Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

230258C5

Cat no : 83070-3-PBS

Synonyms

Adipoq, apM1, adipo, Acrp30, 230258C5

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP00039-3

  Sandwich ELISA standard curve of MP00039-3, MOUSE Adiponectin Recombinant Matched Antibody Pair - PBS only. 83070-6-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0262. 83070-3-PBS was HRP conjugated as the detection antibody. Range:15.6-1000pg/mL.

• 

  Sample test of MP00039-3

  lungs from one mouse were chopped into 1-2 mm pieces and seeded into approximately 30 mL of media containing RPMI, 10% fetal bovine serum, 50 μM β-mercaptoethanol, and L-glutamine.The mean Adiponectin concentration was determined to be 4.4 ng/mL in lung cell culture supernatant.

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  Sample test of MP00039-3

  Organs from 2 mice were rinsed with PBS to remove excess blood, chopped into 1-2 mm pieces, homogenized in 5-10 mL of PBS in a tissue homogenizer, and stored at ≤ -80℃ 5 min. After two freeze-thaw cycles were performed to break the cell membranes, the homogenates were centrifuged for 5 minutes at 5000 x g to remove particulate. The mean Adiponectin concentration was determined to be 130.21 ng/mL in spleen homogenates, 745.9 ng/mL in fat tissue homogenates.

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  Sample test of MP00039-3

  Serum of sixteen mice was measured. The Adiponectin concentration of detected samples was determined to be 7.30 ug/mL with a range of 4.72-13.56 ug/mL.

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Product Information

83070-3-PBS targets Adiponectin as part of a matched antibody pair:

MP00039-3: 83070-6-PBS capture and 83070-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: adiponectin, C1Q and collagen domain containing
• Calculated Molecular Weight: 27kd
• GenBank Accession Number: NM_009605
• Gene Symbol: Adiponectin
• Gene ID (NCBI): 11450
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.