ATRIP Polyclonal antibody

• Featured Product
• KD/KO Validated

ATRIP Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 11327-1-AP

Synonyms

AGS1, ATR interacting protein, ATRIP

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Validation Data Gallery

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  WB analysis using 11327-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11327-1-AP (ATRIP antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11327-1-AP

  WB result of ATRIP antibody (11327-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATRIP transfected HeLa cells.

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  IP experiment of HeLa using 11327-1-AP

  IP result of anti-ATRIP (IP:11327-1-AP, 4ug; Detection:11327-1-AP 1:5000) with HeLa cells lysate 1200 ug.

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells, PC-3 cells
• Positive IP detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below

Product Information

11327-1-AP targets ATRIP in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATRIP fusion protein Ag1869
• Full Name: ATR interacting protein
• Calculated Molecular Weight: 791 aa, 86 kDa
• Observed Molecular Weight: 86-90 kDa
• GenBank Accession Number: BC014153
• Gene Symbol: ATRIP
• Gene ID (NCBI): 84126
• RRID: AB_10837229
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATRIP (ATR Interacting Protein) and ATR signalling complex is a central mediator of the replicative stress response (RSR),and mutations in both genes have been reported in Seckel syndrome.

Publications

Species	Application	Title
mouse	WB,IHC	Biomaterials; A fluorinated peptide with high serum- and lipid-tolerence for the delivery of siRNA drugs to treat obesity and metabolic dysfunction.; Authors - Jin Qiu ; KD Validated
human	WB	Front Pharmacol; Huangkui Capsule Ameliorates Renal Fibrosis in a Unilateral Ureteral Obstruction Mouse Model Through TRPC6 Dependent Signaling Pathways.; Authors - Li-Fei Gu
mouse	WB	Biochem J; Mutation of the RelA(p65) Thr505 phosphosite disrupts the DNA replication stress response leading to CHK1 inhibitor resistance; Authors - Jill E Hunter
mouse	WB	Biochem J; Regulation of CHK1 inhibitor resistance by a c-Rel and USP1 dependent pathway; Authors - Jill E Hunter
human	WB	Cell Insight; ATR/Chk1 interacting lncRNA modulates DNA damage response to induce breast cancer chemoresistance; Authors - Rong Luo