ATP6V1G3 Polyclonal antibody, PBS Only

ATP6V1G3 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 19523-1-PBS

Synonyms

---

Validation Data Gallery

• 

  WB analysis of HEK-293 using 19523-1-AP (same clone as 19523-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19523-1-AP (ATP6V1G3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19523-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse kidney using 19523-1-AP (same clone as 19523-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 19523-1-AP (ATP6V1G3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19523-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse kidney using 19523-1-AP (same clone as 19523-1-PBS)

  IP result of anti-ATP6V1G3 (IP:19523-1-AP, 4ug; Detection:19523-1-AP 1:800) with mouse kidney tissue lysate 4800ug. This data was developed using the same antibody clone with 19523-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 19523-1-AP (same clone as 19523-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 19523-1-AP (ATP6V1G3 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 19523-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 19523-1-AP (same clone as 19523-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 19523-1-AP (ATP6V1G3 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 19523-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 19523-1-AP (same clone as 19523-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 19523-1-AP (ATP6V1G3 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 19523-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 19523-1-AP (same clone as 19523-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 19523-1-AP (ATP6V1G3 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 19523-1-PBS in a different storage buffer formulation.

---

Product Information

19523-1-PBS targets ATP6V1G3 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G3
• Calculated Molecular Weight: 14 kDa
• Observed Molecular Weight: 14 kDa
• GenBank Accession Number: NM_133326
• Gene Symbol: ATP6V1G3
• Gene ID (NCBI): 127124
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ATP6V1G3,also named as ATP6G3, belongs to the V-ATPase G subunit family. ATP6V1G3 is a catalytic subunit of the peripheral V1 complex of vacuolar ATPase (V-ATPase). V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.