ATP6V1A Polyclonal antibody

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  WB analysis using 17115-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17115-1-AP (ATP6V1A antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17115-1-AP

  WB result of ATP6V1A antibody (17115-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATP6V1A transfected HeLa cells.

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  WB analysis of mouse kidney using 17115-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17115-1-AP (ATP6V1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 17115-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17115-1-AP (ATP6V1A antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 17115-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 17115-1-AP (ATP6V1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 17115-1-AP

  IP result of anti-ATP6V1A (IP:17115-1-AP, 4ug; Detection:17115-1-AP 1:500) with HeLa cells lysate 4000ug.

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  IHC staining of human pancreas using 17115-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 17115-1-AP (ATP6V1A antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human pancreas using 17115-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 17115-1-AP (ATP6V1A antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human thyroid cancer using 17115-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 17115-1-AP (ATP6V1A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human thyroid cancer using 17115-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 17115-1-AP (ATP6V1A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 17115-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ATP6V1A antibody (17115-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of NIH/3T3 using 17115-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using ATP6V1A antibody (17115-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• Featured Product
• KD/KO Validated

ATP6V1A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 17115-1-AP

Publications(39)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

V-ATPase subunit A, V-ATPase 69 kDa subunit, VA68, EC:7.1.2.2, ATP6V1A1

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Tested Applications

• Positive WB detected in: A431 cells, human placenta tissue, mouse kidney tissue, HeLa cells, Daudi cells, HepG2 cells, K-562 cells, U-87 MG cells, mouse testis tissue, rat testis tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human pancreas tissue, human thyroid cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: NIH/3T3 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 29 publications below
• IHC: See 2 publications below
• IF: See 11 publications below
• IP: See 2 publications below

Product Information

17115-1-AP targets ATP6V1A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, monkey, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATP6V1A fusion protein Ag10801
• Full Name: ATPase, H+ transporting, lysosomal 70kDa, V1 subunit A
• Calculated Molecular Weight: 617 aa, 68 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC013138
• Gene Symbol: ATP6V1A
• Gene ID (NCBI): 523
• RRID: AB_2290195
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P38606
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The vacuolar-type H(+)-ATPase (V-ATPase) is responsible for the acidification of endosomes, lysosomes, and other intracellular organelles. It is also involved in hydrogen ion transport across the plasma membrane into the extracellular space. The V-ATPase is a multisubunit complex with cytosolic and transmembrane domains. The cytosolic catalytic domain consists of 3 A subunits and 3 B subunits, which bind and hydrolyze ATP, as well as regulatory accessory subunits.ATP6V1A is V-type proton ATPase catalytic subunit A.

Publications

Species	Application	Title
human	WB	Cell; Identification of Required Host Factors for SARS-CoV-2 Infection in Human Cells.; Authors - Zharko Daniloski
mouse	WB	Nat Immunol; Heme drives hemolysis-induced susceptibility to infection via disruption of phagocyte functions.; Authors - Rui Martins
human	WB	Nat Microbiol; The interferon-inducible isoform of NCOA7 inhibits endosome-mediated viral entry.; Authors - Tomas Doyle
human	WB	Autophagy; Follicular lymphoma-associated mutations in the V-ATPase chaperone VMA21 activate autophagy creating a targetable dependency; Authors - Fangyang Wang
human,mouse	WB	Mol Psychiatry; Sphingomyelin-induced inhibition of the plasma membrane calcium ATPase causes neurodegeneration in type A Niemann-Pick disease.; Authors - A Pérez-Cañamás
human	WB	Mol Ther; Caspase-8 Induces Lysosome-Associated Cell Death in Cancer Cells.; Authors - Benfu Zhong

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Paulina (Verified Customer) (03-26-2025)

Fixation: 2% PFA for 20min. Permeabilization/antibody dilution solution: 0.05% saponin, 5% horse serum in PBS. Primary antibody diluted 1:100 in saponin solution for 1hr at room temperature, followed by anti-rabbit conjugated to AlexaFluor 488 (1:200 in saponin solution) for 1 hr at room temperature.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: MDA-MB-231 cells

ATP6V1A Antibody Immunofluorescence validation (1:100 dilution) in MDA-MB-231 cells (Cat no:17115-1-AP)