ATP6 Polyclonal antibody

ATP6 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

52

Cat no : 55313-1-AP

Synonyms

MT-ATP6, MTATP6, MT ATP6, ATPASE6, ATP 6

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Validation Data Gallery

• 

  WB analysis using 55313-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55313-1-AP (ATP6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of C2C12 using 55313-1-AP

  C2C12 cell were subjected to SDS PAGE followed by western blot with 55313-1-AP (ATP6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse brain tissue, C2C12 cells, rat brain

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 51 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

55313-1-AP targets ATP6 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: ATP synthase 6; ATPase subunit 6
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 25-30 kDa
• GenBank Accession Number: J01415
• Gene Symbol: ATP6
• Gene ID (NCBI): 4508
• RRID: AB_2881305
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATP synthase, also known as FoF1 complex, is a critical mitochondrial OXPHOS enzyme involved in the regulation of mitochondrial ATP production and in the maintenance of the mitochondrial membrane potential. It is composed of three components (F1, Fo and the peripheral stalk). F1, the soluble catalytic core, is above the membrane, inside the matrix of the mitochondria; consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon); Fo, comprising the proton channel, is within the membrane; Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). ATP6 is the a subunit of Fo region.

Publications

Species	Application	Title
mouse	WB	Nat Struct Mol Biol; TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS.; Authors - Xinxin Zuo
human	WB	Nat Struct Mol Biol; Single-nucleoid architecture reveals heterogeneous packaging of mitochondrial DNA; Authors - R Stefan Isaac
human	WB	Mol Cell; Base-Resolution Mapping Reveals Distinct m1A Methylome in Nuclear- and Mitochondrial-Encoded Transcripts.; Authors - Xiaoyu Li
human	WB	Nat Commun; RNA modification landscape of the human mitochondrial tRNALys regulates protein synthesis.; Authors - Uwe Richter
human	WB	Nucleic Acids Res; A coronary artery disease-associated tRNAThr mutation altered mitochondrial function, apoptosis and angiogenesis.; Authors - Zidong Jia
human, mouse	WB	Nat Commun; Deregulation of mitochondrial F1FO-ATP synthase via OSCP in Alzheimer's disease.; Authors - Simon J Beck

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