ATP5O Polyclonal antibody

ATP5O Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 10994-1-AP

Synonyms

ATP5PO, OSCP, ATPO, ATP synthase peripheral stalk subunit OSCP

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Validation Data Gallery

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  WB analysis using 10994-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10994-1-AP (ATP5O antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 10994-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 10994-1-AP (OSCP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse placenta using 10994-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 10994-1-AP (OSCP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 10994-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10994-1-AP (OSCP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 10994-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10994-1-AP (OSCP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 10994-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10994-1-AP (ATP5O antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10994-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10994-1-AP (ATP5O antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10994-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10994-1-AP (ATP5O antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 10994-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10994-1-AP (OSCP antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 10994-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10994-1-AP (OSCP antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 10994-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 10994-1-AP (OSCP antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 10994-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 10994-1-AP (OSCP antibody) at dilution of 1:50 (under 40x lens).

• 

  FC experiment of HepG2 using 10994-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug ATP5O Polyclonal antibody (10994-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: A549 cells, mouse placenta tissue, mouse liver tissue, mouse heart tissue, mouse colon tissue, Caco-2 cells, HCT116 cells, HEK-293 cells, HeLa cells, HepG2 cells
• Positive IHC detected in: human lung cancer tissue, human colon cancer tissue, human testis tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below
• IHC: See 1 publications below

Product Information

10994-1-AP targets ATP5O in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATP5O fusion protein Ag1458
• Full Name: ATP synthase, H+ transporting, mitochondrial F1 complex, O subunit
• Calculated Molecular Weight: 23 kDa
• Observed Molecular Weight: 23-25 kDa
• GenBank Accession Number: BC021233
• Gene Symbol: ATP5O
• Gene ID (NCBI): 539
• RRID: AB_2062063
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATP5O(ATP synthase subunit O, mitochondrial) is also named as ATPO, OSCP and belongs to the ATPase delta chain family. The gene encodes the oligomycin sensitivity-conferring protein (OSCP) of ATP synthase. The predicted polypeptide is 213 amino acids long with more than 80% identity to the bovine and mouse homologs and it is expressed at highest levels in muscle and heart by the northern blot(PMID:7490082). Many studies have indicated that mitochondrial dysfunction is central to the development of most age-related human diseases including neurodegenerative diseases, cancer, and type 2 diabetes.

Publications

Species	Application	Title
human	IHC	Cancers (Basel); Proteomic Analysis Identifies NDUFS1 and ATP5O as Novel Markers for Survival Outcome in Prostate Cancer.; Authors - Robert Wiebringhaus
human	WB	Neural Regen Res; CHCHD2 Thr61Ile mutation impairs F1F0-ATPase assembly in in vitro and in vivo models of Parkinson's disease; Authors - Xiang Chen
human	WB	Brain Pathol; Fatal familial insomnia: mitochondrial and protein synthesis machinery decline in the mediodorsal thalamus.; Authors - Margalida A Frau-Méndez
human	WB	J Mol Biol; COA6 Facilitates Cytochrome c Oxidase Biogenesis as Thiol-reductase for Copper Metallochaperones in Mitochondria.; Authors - David Pacheu-Grau
human	WB	Dis Markers; CYC1 Predicts Poor Prognosis in Patients with Breast Cancer.; Authors - Yingyan Han
		Oncol Lett; Lentivirus-mediated silencing of MPHOSPH8 inhibits MTC proliferation and enhances apoptosis.; Authors - Peiyong Li