ATG14/Barkor (C-terminal) Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

ATG14/Barkor (C-terminal) Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 24412-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of HeLa using 24412-1-AP (same clone as 24412-1-PBS)

  WB result of ATG14/Barkor (C-terminal) antibody (24412-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATG14/Barkor (C-terminal) transfected HeLa cells. This data was developed using the same antibody clone with 24412-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 24412-1-AP (same clone as 24412-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 24412-1-AP (ATG14/Barkor (C-terminal) antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24412-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 24412-1-AP (same clone as 24412-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain slide using 24412-1-AP (ATG14/Barkor (C-terminal) antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 24412-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 24412-1-AP (same clone as 24412-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain slide using 24412-1-AP (ATG14/Barkor (C-terminal) antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 24412-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 24412-1-AP (same clone as 24412-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver slide using 24412-1-AP (ATG14/Barkor (C-terminal) antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 24412-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 24412-1-AP (same clone as 24412-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver slide using 24412-1-AP (ATG14/Barkor (C-terminal) antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 24412-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 24412-1-AP (same clone as 24412-1-PBS)

  Immunofluorescent analysis of HeLa cells using 24412-1-AP (ATG14/Barkor (C-terminal) antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG. This data was developed using the same antibody clone with 24412-1-PBS in a different storage buffer formulation.

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Product Information

24412-1-PBS targets ATG14/Barkor (C-terminal) in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATG14/Barkor (C-terminal) fusion protein Ag19707
• Full Name: KIAA0831
• Calculated Molecular Weight: 492 aa, 55 kDa
• Observed Molecular Weight: 65-68 kDa, 42 kDa
• GenBank Accession Number: BC109090
• Gene Symbol: ATG14
• Gene ID (NCBI): 22863
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Barkor, also named as ATG14, Atg14L and KIAA0831, is named as Beclin 1-associated autophagy-related key regulator protein. The function of Barkor in autophagy has been manifested in several assays, including stress-induced LC3 lipidation, autophagosome formation, and Salmonella typhimurium amplification. A major band around 65-68 kDa and a light band around 42 kDa can be observed in western blot which may be caused by alternative splicing of ATG14. The specificity of ATG14 antibody has been tested by siRNA test.