ATF2-Specific Polyclonal antibody

• Featured Product
• KD/KO Validated

ATF2-Specific Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 14834-1-AP

Synonyms

ATF2, ATF2-Specific, CRE BP1, CREB 2, CREB2, CREBP1, HB16, TREB7

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Validation Data Gallery

• 

  WB analysis of HeLa using 14834-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14834-1-AP (ATF2-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C2C12 using 14834-1-AP

  C2C12 cell were subjected to SDS PAGE followed by western blot with 14834-1-AP (ATF2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of LNCaP using 14834-1-AP

  LNCaP cells were subjected to SDS PAGE followed by western blot with 14834-1-AP (ATF2-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 14834-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14834-1-AP (ATF2-Specific antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 14834-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14834-1-AP (ATF2-Specific antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 14834-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14834-1-AP (ATF2-Specific antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 14834-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14834-1-AP (ATF2-Specific antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, LNCaP cells, C2C12 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

14834-1-AP targets ATF2-Specific in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: activating transcription factor 2
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC026175
• Gene Symbol: ATF2
• Gene ID (NCBI): 1386
• RRID: AB_10694139
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATF2, also named as CREB2 and CREBP1, contains one bZIP domain and one C2H2-type zinc finger. It belongs to the bZIP family. ATF2 binds to the cAMP-responsive element(CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. It is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. ATF2 binds DNA as a dimer and can form a homodimer in the absence of DNA. It binds through its N-terminal region to UTF1 which acts as a coactivator of ATF2 transcriptional activity. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human ATF2. The calculated molecular weight of ATF2 is 55 kDa, but the modified ATF2 protein is about 70 kDa (PMID: 18269766 ).

Publications

Species	Application	Title
human	WB	Nature; A druggable copper-signalling pathway that drives inflammation; Authors - Stéphanie Solier
mouse	IHC	Nature; Divergent transcriptional regulation of astrocyte reactivity across disorders.; Authors - Joshua E Burda
human	WB	BMC Med; Downregulation of AC092894.1 promotes oxaliplatin resistance in colorectal cancer via the USP3/AR/RASGRP3 axis; Authors - Zhijian Zheng
mouse	WB	Cell Rep; Cold-Inducible SIRT6 Regulates Thermogenesis of Brown and Beige Fat.; Authors - Lu Yao
human	WB	J Biol Chem; MicroRNA-26b Inhibits Hepatitis B Virus Transcription and Replication by Targeting the Host Factor CHORDC1.; Authors - Fanpeng Zhao
human	WB	Artif Cells Nanomed Biotechnol; Molecular mechanism of miR-204 regulates proliferation, apoptosis and autophagy of cervical cancer cells by targeting ATF2.; Authors - Nan Li ; KD Validated

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