ATF1 Polyclonal antibody

• Featured Product
• KD/KO Validated

ATF1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 11946-1-AP

Synonyms

TREB36, Cyclic AMP-dependent transcription factor ATF-1, cAMP-dependent transcription factor ATF-1

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Validation Data Gallery

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  WB analysis of HeLa using 11946-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11946-1-AP (ATF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11946-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11946-1-AP (ATF1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 11946-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 11946-1-AP (ATF1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 11946-1-AP

  IP result of anti-ATF1 (IP:11946-1-AP, 4ug; Detection:11946-1-AP 1:500) with HeLa cells lysate 3200ug.

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  IF Staining of HeLa using 11946-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 11946-1-AP (ATF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, A431 cells, human brain tissue
• Positive IP detected in: HeLa cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 9 publications below
• IHC: See 1 publications below

Product Information

11946-1-AP targets ATF1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATF1 fusion protein Ag2548
• Full Name: activating transcription factor 1
• Calculated Molecular Weight: 271 aa, 29 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC029619
• Gene Symbol: ATF1
• Gene ID (NCBI): 466
• RRID: AB_2060777
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TFs play a vital role in maintaining cell homeostasis by combining DNA regulatory sequences to regulate gene transcription rates. Activating transcription factor 1 (ATF1) is a member of the ATF/CREB family of transcription factors (TFs), specifically interacting with the consensus ATF/CRE site 'TGACGTCA'(PMID: 28032861). ATF1 transcriptionally regulates the expressions of target genes to mediate various cellular processes, and ATF1 effects the progression of tumors (including lung cancer, colorectal cancer,and nasopharyngeal carcinoma).

Publications

Species	Application	Title
human	WB	Am J Hum Genet; Systematic Functional Interrogation of Genes in GWAS Loci Identified ATF1 as a Key Driver in Colorectal Cancer Modulated by a Promoter-Enhancer Interaction.; Authors - Jianbo Tian ; KD Validated
mouse	WB	Mol Ther Nucleic Acids; Mbd2 deficiency alleviates retinal cell apoptosisvia the miR-345-5p/Atf1 axis in high glucoseinjury and streptozotocin-induced diabetic mice.; Authors - Yanni Ge
human	WB	Sci Signal; The death-inducing activity of RIPK1 is regulated by the pH environment.; Authors - Kenta Moriwaki
human	WB	Front Cell Dev Biol; CREB1 and ATF1 Negatively Regulate Glutathione Biosynthesis Sensitizing Cells to Oxidative Stress.; Authors - Lina Zhao ; KD Validated
human	WB	J Biol Chem; The mRNA levels of heat shock factor 1 are regulated by thermogenic signals via the cAMP-dependent transcription factor ATF3.; Authors - Narendra Verma
human	WB	Cells; Perilipin 5 Protects against Cellular Oxidative Stress by Enhancing Mitochondrial Function in HepG2 Cells.; Authors - Yanjie Tan