ATAD4 / PRR15L Polyclonal antibody

ATAD4 / PRR15L Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25693-1-AP

Synonyms

ATAD4, Protein ATAD4, PRR15L

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Validation Data Gallery

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  IHC staining of mouse kidney using 25693-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 25693-1-AP (ATAD4 / PRR15L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 25693-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 25693-1-AP (ATAD4 / PRR15L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 25693-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using ATAD4 / PRR15L antibody (25693-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IHC detected in: mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25693-1-AP targets ATAD4 / PRR15L in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATAD4 / PRR15L fusion protein Ag22500
• Full Name: ATPase family, AAA domain containing 4
• Calculated Molecular Weight: 103 aa, 12 kDa
• GenBank Accession Number: BC002865
• Gene Symbol: ATAD4
• Gene ID (NCBI): 79170
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATAD4 (ATPase family AAA domain-containing protein 4), also known as PRR15L (Proline Rich 15 Like), and its function has not yet been discovered.