ASB13 Polyclonal antibody

ASB13 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25616-1-AP

Synonyms

ASB-13, ASB 13

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Validation Data Gallery

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  WB analysis using 25616-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25616-1-AP (ASB13 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human small intestine using 25616-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25616-1-AP (ASB13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 25616-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25616-1-AP (ASB13 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 25616-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25616-1-AP (ASB13 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon using 25616-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25616-1-AP (ASB13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human small intestine using 25616-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25616-1-AP (ASB13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 25616-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ASB13 antibody (25616-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

• Positive WB detected in: L02 cells, mouse heart tissue, mouse liver tissue, rat heart tissue, rat liver tissue
• Positive IHC detected in: human small intestine tissue, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25616-1-AP targets ASB13 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ASB13 fusion protein Ag22533
• Full Name: ankyrin repeat and SOCS box-containing 13
• Calculated Molecular Weight: 278 aa, 30 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC012056
• Gene Symbol: ASB13
• Gene ID (NCBI): 79754
• RRID: AB_2880162
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.