ARX Polyclonal antibody

ARX Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 20739-1-AP

Synonyms

aristaless related homeobox, ARX, Homeobox protein ARX, ISSX, MRX29, MRX32, MRX33, MRX36, MRX38, MRX43, MRX54, MRX76, MRX87, MRXS1, PRTS

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Validation Data Gallery

• 

  WB analysis of NIH/3T3 using 20739-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 20739-1-AP (ARX Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 20739-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20739-1-AP (ARX Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 20739-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 20739-1-AP (ARX Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 20739-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 20739-1-AP (ARX Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 20739-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20739-1-AP (ARX Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20739-1-AP targets ARX in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: aristaless related homeobox
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: NM_139058
• Gene Symbol: ARX
• Gene ID (NCBI): 170302
• RRID: AB_2878729
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ARX belongs to the paired homeobox family and Bicoid subfamily. It is a transcription factor which required for normal brain development. ARX may be important for maintenance of specific neuronal subtypes in the cerebral cortex and axonal guidance in the floor plate. Defects in ARX are the cause of lissencephaly X-linked type 2 (LISX2), epileptic encephalopathy early infantile type 1 (EIEE1), Partington syndrome (PRTS), mental retardation X-linked ARX-related (MRXARX), and agenesis of the corpus callosum with abnormal genitalia (ACCAG). The antibody is specific to ARX. This is a rabbit polyclonal antibody raised against a peptide mapping within human ARX.