ART1 Monoclonal antibody, PBS Only

ART1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1D10A8

Cat no : 66958-1-PBS

Synonyms

ADP ribosyltransferase 1, ART1, ART2, CD296, Mono(ADP ribosyl)transferase 1, RT6

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Validation Data Gallery

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  WB analysis using 66958-1-Ig (same clone as 66958-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66958-1-Ig (ART1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66958-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66958-1-Ig (same clone as 66958-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66958-1-Ig (ART1 antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66958-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66958-1-Ig (same clone as 66958-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66958-1-Ig (ART1 antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66958-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66958-1-Ig (same clone as 66958-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66958-1-Ig (ART1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66958-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66958-1-Ig (same clone as 66958-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66958-1-Ig (ART1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66958-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66958-1-PBS targets ART1 in WB, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ART1 fusion protein Ag28269
• Full Name: ADP-ribosyltransferase 1
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 34-36 kDa
• GenBank Accession Number: NM_004314
• Gene Symbol: ART1
• Gene ID (NCBI): 417
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

ART1 (ADP-ribosyltransferase 1) is an important mono-ADP-ribose transferase which can catalyze the mono-ADP-ribosylation of proteins including human neutrophil peptide-1 (HNP-1), fibroblast growth factor-2 (FGF-2) and platelet-derived growth factor-BB (PDGF-BB), and play a role in biological processes such as proliferation and invasion of cancer cells.