ART1 Monoclonal antibody

ART1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (2)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1D10A8

Cat no : 66958-1-Ig

Synonyms

ADP ribosyltransferase 1, ART1, ART2, CD296, Mono(ADP ribosyl)transferase 1, RT6



Tested Applications

Positive WB detected inEC109 cells, HeLa cells, HEK-293 cells, HepG2 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1500-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66958-1-Ig targets ART1 in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ART1 fusion protein Ag28269
Full Name ADP-ribosyltransferase 1
Calculated Molecular Weight 36 kDa
Observed Molecular Weight 34-36 kDa
GenBank Accession NumberNM_004314
Gene Symbol ART1
Gene ID (NCBI) 417
RRIDAB_2882281
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ART1 (ADP-ribosyltransferase 1) is an important mono-ADP-ribose transferase which can catalyze the mono-ADP-ribosylation of proteins including human neutrophil peptide-1 (HNP-1), fibroblast growth factor-2 (FGF-2) and platelet-derived growth factor-BB (PDGF-BB), and play a role in biological processes such as proliferation and invasion of cancer cells.

Protocols

Product Specific Protocols
WB protocol for ART1 antibody 66958-1-IgDownload protocol
IHC protocol for ART1 antibody 66958-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Med Oncol

Identification of the role of mono-ADP-ribosylation in colorectal cancer by integrated transcriptome analysis.

Authors - Shuxian Zhang
human,mouseIHC,IF,WB

BMC Cancer

ART1 knockdown decreases the IL-6-induced proliferation of colorectal cancer cells

Authors - Ting Lin
  • KD Validated