ARNT/HIF-1 beta Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

ARNT/HIF-1 beta Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 14105-1-PBS

Synonyms

ARNT, HIF1B, HIF1 beta, HIF 1 beta, Class E basic helix-loop-helix protein 2

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Validation Data Gallery

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  WB analysis of HeLa using 14105-1-AP (same clone as 14105-1-PBS)

  WB result of ARNT/HIF-1 beta antibody (14105-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ARNT,HIF1B transfected HeLa cells. This data was developed using the same antibody clone with 14105-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 14105-1-AP (same clone as 14105-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14105-1-AP (ARNT/HIF-1 beta antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14105-1-PBS in a different storage buffer formulation.

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  WB analysis using 14105-1-AP (same clone as 14105-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14105-1-AP (ARNT/HIF-1 beta antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14105-1-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 14105-1-AP (same clone as 14105-1-PBS)

  IP result of anti-ARNT/HIF-1 beta (IP:14105-1-AP, 4ug; Detection:14105-1-AP 1:1000) with HepG2 cells lysate 3600ug. This data was developed using the same antibody clone with 14105-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse embryo using 14105-1-AP (same clone as 14105-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 14105-1-AP (ARNT/HIF-1 beta Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14105-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse embryo using 14105-1-AP (same clone as 14105-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 14105-1-AP (ARNT/HIF-1 beta Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14105-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse embryo using 14105-1-AP (same clone as 14105-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 14105-1-AP (ARNT/HIF-1 beta Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14105-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 14105-1-AP (same clone as 14105-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 14105-1-AP (ARNT/HIF-1 beta antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14105-1-PBS in a different storage buffer formulation.

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Product Information

14105-1-PBS targets ARNT/HIF-1 beta in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ARNT/HIF-1 beta fusion protein Ag5238
• Full Name: aryl hydrocarbon receptor nuclear translocator
• Calculated Molecular Weight: 416 aa, 46 kDa, 87 kDa
• Observed Molecular Weight: 86 kDa
• GenBank Accession Number: BC060838
• Gene Symbol: ARNT/HIF-1 beta
• Gene ID (NCBI): 405
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

HIF1B(HIF-1 beta), also named as ARNT, is a 789 amino acid protein, which forms a heterodimer with AHR, AHRR, HIF1A and EPAS1/HIF2A as well as with other bHLH proteins. HIF1B is Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia