ARNT/HIF-1 beta Polyclonal antibody

• Featured Product
• KD/KO Validated

ARNT/HIF-1 beta Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

20

Cat no : 14105-1-AP

Synonyms

ARNT, HIF1B, HIF1 beta, HIF 1 beta, Class E basic helix-loop-helix protein 2

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Validation Data Gallery

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  WB analysis of HeLa using 14105-1-AP

  WB result of ARNT/HIF-1 beta antibody (14105-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ARNT,HIF1B transfected HeLa cells.

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  WB analysis of HepG2 using 14105-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14105-1-AP (ARNT/HIF-1 beta antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 14105-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14105-1-AP (ARNT/HIF-1 beta antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 14105-1-AP

  IP result of anti-ARNT/HIF-1 beta (IP:14105-1-AP, 4ug; Detection:14105-1-AP 1:1000) with HepG2 cells lysate 3600ug.

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  IHC staining of mouse embryo using 14105-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 14105-1-AP (ARNT/HIF-1 beta Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse embryo using 14105-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 14105-1-AP (ARNT/HIF-1 beta Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse embryo using 14105-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 14105-1-AP (ARNT/HIF-1 beta Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 14105-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 14105-1-AP (ARNT/HIF-1 beta antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: mouse embryo tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 17 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 2 publications below

Product Information

14105-1-AP targets ARNT/HIF-1 beta in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ARNT/HIF-1 beta fusion protein Ag5238
• Full Name: aryl hydrocarbon receptor nuclear translocator
• Calculated Molecular Weight: 416 aa, 46 kDa, 87 kDa
• Observed Molecular Weight: 86 kDa
• GenBank Accession Number: BC060838
• Gene Symbol: ARNT/HIF-1 beta
• Gene ID (NCBI): 405
• RRID: AB_10639523
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HIF1B(HIF-1 beta), also named as ARNT, is a 789 amino acid protein, which forms a heterodimer with AHR, AHRR, HIF1A and EPAS1/HIF2A as well as with other bHLH proteins. HIF1B is Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia

Publications

Species	Application	Title
human	WB	Hepatology; PROX1 promotes hepatocellular carcinoma metastasis by way of up-regulating hypoxia-inducible factor 1α expression and protein stability.; Authors - Liu Yanfeng Y
human	WB,IF	BMC Med; NUPR1 contributes to radiation resistance by maintaining ROS homeostasis via AhR/CYP signal axis in hepatocellular carcinoma; Authors - Yizhi Zhan
human	WB	Free Radic Biol Med; Sestrin2 inhibits hypoxia-inducible factor-1α accumulation via AMPK-mediated prolyl hydroxylase regulation.; Authors - Kyuhwa Seo
mouse	WB	Cell Immunol; Fine particulate matter (PM2.5) induces inhibitory memory alveolar macrophages through the AhR/IL-33 pathway; Authors - Yanan Liu
mouse	WB	Front Microbiol; Fine Particulate Matter (PM2.5) Promotes CD146 Expression in Alveolar Epithelial Cells and Cryptococcus neoformans Pulmonary Infection.; Authors - Zhixiao Sun
mouse	WB	J Biomed Nanotechnol; Fine Particulate Matter (PM2.5) Promoted the Invasion of Lung Cancer Cells via an ARNT2/PP2A/STAT3/MMP2 Pathway.; Authors - Zhongqi Chen