ARMC9 Polyclonal antibody

ARMC9 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 28001-1-AP

Synonyms

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Validation Data Gallery

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  WB analysis using 28001-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 28001-1-AP (ARMC9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of hTERT-RPE1 using 28001-1-AP

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using ARMC9 antibody (28001-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CoraLite®594 ARL13B antibody (CL594-17711, red).

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Tested Applications

• Positive WB detected in: human placenta tissue, mouse placenta tissue, mouse retina tissue, rat retina tissue
• Positive IF/ICC detected in: hTERT-RPE1 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28001-1-AP targets ARMC9 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ARMC9 fusion protein Ag27511
• Full Name: armadillo repeat containing 9
• Calculated Molecular Weight: 817 aa, 92 kDa
• Observed Molecular Weight: 76-78 kDa
• GenBank Accession Number: BC004514
• Gene Symbol: ARMC9
• Gene ID (NCBI): 80210
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ARMC9 (Armadillo Repeat Containing 9) belongs to the ARMC subfamily characterized by a class of tandem repeat proteins with a length of approximately 42 amino acids (PMID: 34912852). ARMC9 localizes to centrioles and the proximal portion of the cilium in mammalian cilia (PMID: 32453716). The variants of ARMC9 leads to Joubert syndrome (JBTS) ,a recessive neurodevelopmental ciliopathy characterized by characterized by hypotonia, ataxia, abnormal eye movements, and variable cognitive impairment(PMID: 28625504; PMID: 32453716). ARMC9 has two isoforms with molecular weights of 92 kDa and 76 kDa.