ARHGAP28 Polyclonal antibody

ARHGAP28 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 26766-1-AP

Synonyms

KIAA1314, cDNA FLJ58638, highly similar to Homo sapiens Rho GTPase activating protein 28

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Validation Data Gallery

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  WB analysis using 26766-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26766-1-AP (ARHGAP28 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 26766-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26766-1-AP (ARHGAP28 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 26766-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26766-1-AP (ARHGAP28 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: C2C12 cells, mouse testis tissue
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

26766-1-AP targets ARHGAP28 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ARHGAP28 fusion protein Ag25293
• Full Name: Rho GTPase activating protein 28
• Observed Molecular Weight: 82-90kDa
• GenBank Accession Number: BC065274
• Gene Symbol: ARHGAP28
• Gene ID (NCBI): 79822
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ARHGAP28 (Rho GTPase Activating Protein 28), also known as FLJ10312 and KIAA1314, is a Rho GTPase activating protein (RhoGAP) that switches RhoA to its inactive form. It is reported that high expression of ARHGAP28 in osteosarcoma cell lines can inhibit the proliferation, migration, and invasion of osteosarcoma(PMID: 38041020).

Publications

Species	Application	Title
human	WB	Clin Exp Pharmacol Physiol; ARHGAP17 inhibits pathological cyclic strain-induced apoptosis in human periodontal ligament fibroblasts via Rac1/Cdc42.; Authors - Li Wang