Arginase-2 Polyclonal antibody

• Featured Product
• KD/KO Validated

Arginase-2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

10

Cat no : 14825-1-AP

Synonyms

ARG2, ARG 2, EC:3.5.3.1, Arginase-2, mitochondrial, arginase, type II

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Validation Data Gallery

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  WB analysis of Caco-2 using 14825-1-AP

  WB result of ARG2 antibody (14825-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ARG2 transfected Caco-2 cells.

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  WB analysis of HEK-293 using 14825-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14825-1-AP (ARG2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 14825-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14825-1-AP (ARG2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 14825-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14825-1-AP (ARG2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 14825-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14825-1-AP (ARG2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 14825-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14825-1-AP (ARG2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate cancer using 14825-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14825-1-AP (ARG2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, Caco-2 cells
• Positive IHC detected in: human prostate cancer tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Product Information

14825-1-AP targets Arginase-2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Arginase-2 fusion protein Ag6609
• Full Name: arginase, type II
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 39-42 kDa
• GenBank Accession Number: BC001350
• Gene Symbol: ARG2
• Gene ID (NCBI): 384
• RRID: AB_2058977
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Arginase 2 is composed of 354 amino acid residues, including an NH2-terminal presequence for mitochondrial targeting and import. In the mitochondria, ornithine generated by Arginase 2 will give rise to glutamate via ornithine aminotransferase (OAT). Glutamate participates in several transamination reactions, including forming α-ketoglutarate (αKG) that may enter the TCA cycle and increase cycle intermediates and flux.Arginase 1 is mainly expressed in hepatocytes, and mice with a disruption of Arginase 1 gene die soon after birth. Arginase 2 is poorly expressed in hepatocytes, and most highly expressed in kidney, prostate, and immune cells such as monocyte/ macrophages. (PMID: 25234945,PMID: 27214549)

Publications

Species	Application	Title
mouse	IF	Circulation; Deletion of Macrophage Low-Density Lipoprotein Receptor-Related Protein 1 (LRP1) Accelerates Atherosclerosis Regression and Increases C-C Chemokine Receptor Type 7 (CCR7) Expression in Plaque Macrophages.; Authors - Paul A Mueller
mouse	WB	J Lipid Res; Macrophage-derived apoESendai suppresses atherosclerosis while causing lipoprotein glomerulopathy in hyperlipidemic mice.; Authors - Hagai Tavori
mouse	WB	Biomed Pharmacother; PMID: 30889486; Authors - Lu Gao
mouse	WB	Biochim Biophys Acta; Reduced bioavailable manganese causes striatal urea cycle pathology in Huntington's disease mouse model.; Authors - Terry Jo V Bichell
	IHC	Biol Reprod; Progesterone and interferon tau regulate expression of polyamine enzymes during the ovine peri-implantation period.; Authors - Katherine M Halloran
human	WB	Life Sci; Ornithine is a key mediator in hyperphosphatemia-mediated human umbilical vein endothelial cell apoptosis: Insights gained from metabolomics.; Authors - Rong Zhou