APOF Polyclonal antibody

APOF Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 16608-1-AP

Synonyms

Apo F, APOF, apolipoprotein F, LTIP

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Validation Data Gallery

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  WB analysis of human plasma using 16608-1-AP

  human plasma were subjected to SDS PAGE followed by western blot with 16608-1-AP (APOF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 16608-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16608-1-AP (APOF antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 16608-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16608-1-AP (APOF antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 16608-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16608-1-AP (APOF antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: human plasma tissue, HepG2 cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

16608-1-AP targets APOF in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: APOF fusion protein Ag9824
• Full Name: apolipoprotein F
• Calculated Molecular Weight: 326 aa, 35 kDa
• Observed Molecular Weight: 30-33 kDa
• GenBank Accession Number: BC026257
• Gene Symbol: APOF
• Gene ID (NCBI): 319
• RRID: AB_2058253
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	Cancer Cell Int; Bioinformatics combined with quantitative proteomics analyses and identification of potential biomarkers in cholangiocarcinoma.; Authors - Zijian Da