APEX1 Polyclonal antibody

• Featured Product
• KD/KO Validated

APEX1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

24

Cat no : 10203-1-AP

Synonyms

APEX nuclease, APEX, APEN, APE-1, APE1

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Validation Data Gallery

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  WB analysis of HeLa using 10203-1-AP

  WB result of APEX1 antibody (10203-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-APEX1 transfected HeLa cells.

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  WB analysis of HeLa using 10203-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10203-1-AP (APEX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 10203-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10203-1-AP (APEX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 10203-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10203-1-AP (APEX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10203-1-AP

  IP result of anti-APEX1 (IP:10203-1-AP, 4ug; Detection:10203-1-AP 1:1000) with HeLa cells lysate 1600ug.

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  IHC staining of rat colon using 10203-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 10203-1-AP (APEX1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 10203-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10203-1-AP (APEX1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human thyroid cancer using 10203-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10203-1-AP (APEX1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 10203-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 10203-1-AP (APEX1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 10203-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using APEX1 antibody (10203-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse brain tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human stomach cancer tissue, human thyroid cancer tissue, mouse colon tissue, rat colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 7 publications below
• WB: See 19 publications below
• IHC: See 5 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

10203-1-AP targets APEX1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: APEX1 fusion protein Ag0251
• Full Name: APEX nuclease (multifunctional DNA repair enzyme) 1
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC002338
• Gene Symbol: APEX1
• Gene ID (NCBI): 328
• RRID: AB_2057927
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

APEX1, also named as APE, APE1, HAP1 and REF-1, belongs to the DNA repair enzymes AP/ExoA family. It is a multifunctional protein that plays a central role in the cellular response to oxidative stress. The two major activities of APEX1 are in DNA repair and redox regulation of transcriptional factors. APEX nuclease is a DNA repair enzyme having apurinic/apyrimidinic (AP) endonuclease, 3-prime,5-prime-exonuclease, DNA 3-prime repair diesterase, and DNA 3-prime-phosphatase activities. On the other hand, APEX1 also exerts reversible nuclear redox activity to regulate DNA binding affinity and transcriptional activity of transcriptional factors by controlling the redox status of their DNA-binding domain, such as the FOS/JUN AP-1 complex after exposure to IR. APEX1 is involved in calcium-dependent down-regulation of parathyroid hormone (PTH) expression by binding to negative calcium response elements (nCaREs). When acetylated at Lys-6 and Lys-7, APEX1 stimulates the YBX1-mediated MDR1 promoter activity, leading to drug resistance. It also acts as an endoribonuclease involved in the control of single-stranded RNA metabolism. It plays a role in regulating MYC mRNA turnover by preferentially cleaving in between UA and CA dinucleotides of the MYC coding region determinant (CRD). In association with NMD1, APEX1 plays a role in the rRNA quality control process during cell cycle progression. 10203-1-AP is a rabbit polyclonal antibody raised against full length APE1 of human origin.

Publications

Species	Application	Title
human	WB	Sci Adv; Deficiency in mammalian STN1 promotes colon cancer development via inhibiting DNA repair; Authors - Dinh Duc Nguyen
human	WB,IHC	Mol Ther; miR-1293, a Candidate for miRNA-Based Cancer Therapeutics, Simultaneously Targets BRD4 and the DNA Repair Pathway.; Authors - Yuki Takagawa ; KD Validated
rat	IF	Free Radic Biol Med; Systematical exploration of transcription factors in cardiomyocytes against hydrogen peroxide-induced oxidative stress by Danhong injection.; Authors - Jingjing Zhang
human	WB,IP	Int Immunopharmacol; Inhibitors of APE1 redox and ATM synergistically sensitize osteosarcoma cells to ionizing radiation by inducing ferroptosis; Authors - Hanxi Xiao ; KD Validated
mouse	WB	Mol Cell Biol; Apurinic/apyrimidinic endonuclease 1 is the essential nuclease during immunoglobulin class switch recombination.; Authors - Masani Shahnaz S
rat,human	IF	Sci Rep; Screening and identification of critical transcription factors involved in the protection of cardiomyocytes against hydrogen peroxide-induced damage by Yixin-shu.; Authors - Jingjing Zhang