AP1B1 Polyclonal antibody

AP1B1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 16932-1-AP

Synonyms

ADTB1, AP 1 complex subunit beta 1, AP105A, AP1B1, BAM22, Beta 1 adaptin, Beta adaptin 1, CLAPB2

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Validation Data Gallery

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  WB analysis of HEK-293 using 16932-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16932-1-AP (AP1B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 16932-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16932-1-AP (AP1B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 16932-1-AP

  IP result of anti-AP1B1 (IP:16932-1-AP, 4ug; Detection:16932-1-AP 1:300) with HEK-293 cells lysate 2320 ug.

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  IF Staining of HepG2 using 16932-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 16932-1-AP (AP1B1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells
• Positive IP detected in: HEK-293 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below

Product Information

16932-1-AP targets AP1B1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AP1B1 fusion protein Ag10417
• Full Name: adaptor-related protein complex 1, beta 1 subunit
• Calculated Molecular Weight: 919 aa, 101 kDa
• Observed Molecular Weight: 101 kDa
• GenBank Accession Number: BC046242
• Gene Symbol: AP1B1
• Gene ID (NCBI): 162
• RRID: AB_2058204
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AP1B1 is a subunit of the adaptor complex AP-1 and is a member of the adaptin protein family. Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP-1 is found at the cytoplasmic face of coated vesicles located at the Golgi complex, where it mediates both the recruitment of clathrin to the membrane and the recognition of sorting signals within the cytosolic tails of transmembrane receptors.

Publications

Species	Application	Title
human	WB	Nat Genet; Bidirectional genome-wide CRISPR screens reveal host factors regulating SARS-CoV-2, MERS-CoV and seasonal HCoVs; Authors - Antoine Rebendenne
human	WB	Am J Hum Genet; Recessive Mutations in AP1B1 Cause Ichthyosis, Deafness, and Photophobia.; Authors - Lynn M Boyden
human	WB	J Cell Biol; Cargo-selective SNX-BAR proteins mediate retromer trimer independent retrograde transport.; Authors - Arunas Kvainickas
human	WB	Oncogene; Regulated secretion of mutant p53 negatively affects T lymphocytes in the tumor microenvironment; Authors - Xiang Dong
human	WB	Sci Adv; ER-export and ARFRP1/AP-1-dependent delivery of SARS-CoV-2 Envelope to lysosomes controls late stages of viral replication; Authors - Guy J Pearson

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