AP repeat Polyclonal antibody

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  ELISA experiment of peptide using 24493-1-AP

  AP repeat Antibody (24493-1-AP) tested in ELISA. AP repeat peptide was coated onto the microplates at 1.5 µg/well and then incubated with dilution series of AP repeat antibody (24493-1-AP). Bound antibodies were detected with HRP conjugated anti-Rabbit IgG followed by incubation with HRP Substrate and then measuring the resulting absorbance at 450 nm.

AP repeat Polyclonal Antibody for ELISA

Cat No. 24493-1-AP

Publications(7)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Synonyms

AP repeat, APAPAPAPA, C9RAN, GGGCC, poly AP

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Tested Applications

• Positive ELISA detected in: peptide

Recommended dilution

• Enzyme-linked Immunosorbent Assay (ELISA): ELISA : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 3 publications below
• IF: See 3 publications below

Product Information

24493-1-AP targets AP repeat in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: AP repeat
• RRID: AB_2879572
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The C9orf72 "GGGGCC" repeat sequence codes five repeat peptide "GA repeat; GAGAGAGAGA", "GP repeat; GPGPGPGPG", "GR repeat; GRGRGRGRG", "AP repeat; APAPAPAPA" and "PR repeat; PRPRPRPRP". It was described previously that aggregated forms of poly-GA and poly-GP proteins do not enter the separation gel (PMID: 26374446). This antibody is used to detect the "AP repeat" sequence. This antibody detects the APAPAPAPA peptide with dilution 1:32,000 in Elisa.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IHC	Acta Neuropathol; Increased prevalence of granulovacuolar degeneration in C9orf72 mutation.; Authors - Yuichi Riku
human	WB, IHC	Acta Neuropathol; Quantitative analysis and clinico-pathological correlations of different dipeptide repeat protein pathologies in C9ORF72 mutation carriers.; Authors - Ian R A Mackenzie
human	IF	Acta Neuropathol; Antisense RNA foci in the motor neurons of C9ORF72-ALS patients are associated with TDP-43 proteinopathy.; Authors - Johnathan Cooper-Knock
human	WB,IF	Hum Mol Genet; Modelling C9orf72 dipeptide repeat proteins of a physiologically relevant size.; Authors - Janis Bennion Callister
human	WB	J Cell Sci; SFPQ regulates the accumulation of RNA foci and dipeptide repeat proteins from the expanded repeat mutation in C9orf72.; Authors - Mirjana Malnar
human	WB	Life Sci Alliance; C9ORF72-derived poly-GA DPRs undergo endocytic uptake in iAstrocytes and spread to motor neurons.; Authors - Paolo M Marchi