Annexin VI Polyclonal antibody

• Featured Product
• KD/KO Validated

Annexin VI Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

10

Reviews (1)

Cat no : 12542-1-AP

Synonyms

Annexin A6, ANXA6, ANX6, Annexin-6, Annexin 6

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Validation Data Gallery

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  WB analysis of HeLa using 12542-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12542-1-AP (Annexin VI antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12542-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12542-1-AP (Annexin VI antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12542-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12542-1-AP (Annexin VI antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12542-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12542-1-AP (Annexin VI antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12542-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12542-1-AP (Annexin VI antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse placenta using 12542-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 12542-1-AP (Annexin VI antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 12542-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12542-1-AP (Annexin VI antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 12542-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12542-1-AP (Annexin VI antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 12542-1-AP

  IP result of anti-Annexin VI (IP:12542-1-AP, 4ug; Detection:12542-1-AP 1:500) with mouse kidney tissue lysate 4000ug.

• 

  IHC staining of human colon using 12542-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12542-1-AP (Annexin VI antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon using 12542-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12542-1-AP (Annexin VI antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human normal colon using 12542-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 12542-1-AP (Annexin VI antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 12542-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12542-1-AP (Annexin VI antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human testis using 12542-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12542-1-AP (Annexin VI antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human kidney using 12542-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12542-1-AP (Annexin VI antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 12542-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12542-1-AP (Annexin VI antibody at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HeLa using 12542-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 12542-1-AP (Annexin VI antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, human brain tissue, mouse brain tissue, mouse placenta tissue, mouse kidney tissue, A549 cells, Jurkat cells
• Positive IP detected in: mouse kidney tissue
• Positive IHC detected in: human colon tissue, human kidney tissue, human normal colon, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Product Information

12542-1-AP targets Annexin VI in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Annexin VI fusion protein Ag3263
• Full Name: annexin A6
• Calculated Molecular Weight: 673 aa, 76 kDa
• Observed Molecular Weight: 67 kDa
• GenBank Accession Number: BC017046
• Gene Symbol: Annexin VI
• Gene ID (NCBI): 309
• RRID: AB_2057753
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ANXA6, also called CBP68, or 67 kDa calelectrin, belongs to a family of calcium-dependent membrane and phospholipid binding proteins. Several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. The annexin VI gene is approximately 60 kbp long and contains 26 exons. It encodes a protein of about 68 kDa that consists of eight 68-amino acid repeats separated by linking sequences of variable lengths. It is highly similar to human annexins I and II sequences, each of which contain four such repeats. Annexin VI has been implicated in mediating the endosome aggregation and vesicle fusion in secreting epithelia during exocytosis.

Publications

Species	Application	Title
mouse	WB	J Extracell Vesicles; Distinguishing functional exosomes and other extracellular vesicles as a nucleic acid cargo by the anion-exchange method.; Authors - Naohiro Seo
human	WB	Oncotarget; CD300A promotes tumor progression by PECAM1, ADCY7 and AKT pathway in acute myeloid leukemia.; Authors - Xiaogang Sun
rat	WB	Front Cell Dev Biol; Dynamic Mitochondrial Proteome Under Polyamines Treatment in Cardiac Aging.; Authors - Hao Zhang
rat	WB,IF	Proteome Sci; TMT-based proteomics analysis of the cerebral cortex of TauT knockout rats.; Authors - Yiming Xia
rat	WB	Proteome Sci; A dynamic plasma membrane proteome analysis of alcohol-induced liver cirrhosis.; Authors - Jia Xiaofang X
human	WB	Onco Targets Ther; UNBS5162 inhibits proliferation of human retinoblastoma cells by promoting cell apoptosis.; Authors - Bing Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Gaurav (Verified Customer) (02-29-2024)

Very good antibody with expected band at appropriate position. Works well in TBST, 5% Skimmed milk and 3% BSA in TBST

• Applications: Western Blot, Immunoprecipitation, Cell culture
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: Smooth Muscle cells, Aorta, Placental Trophoblasts.