ANKRD39 Polyclonal antibody

ANKRD39 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24027-1-AP

Synonyms

ANKRD39, ankyrin repeat domain 39

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Validation Data Gallery

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  WB analysis of PC-3 using 24027-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 24027-1-AP (ANKRD39 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Transfected HEK-293 using 24027-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 24027-1-AP (ANKRD39 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of PC-3 using 24027-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 24027-1-AP (ANKRD39 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: PC-3 cells, Transfected HEK-293 cells
• Positive IF/ICC detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24027-1-AP is ANKRD39 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ANKRD39 fusion protein Ag21210
• Full Name: ankyrin repeat domain 39
• Calculated Molecular Weight: 183 aa, 20 kDa
• Observed Molecular Weight: 20-25 kDa
• GenBank Accession Number: BC031303
• Gene Symbol: ANKRD39
• Gene ID (NCBI): 51239
• RRID: AB_2879407
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.