AMFR/GP78 Polyclonal antibody

• Featured Product
• KD/KO Validated

AMFR/GP78 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

24

Cat no : 16675-1-AP

Synonyms

AMF receptor, isoform 2, AMFR, GP78, RING finger protein 45, RNF45

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Validation Data Gallery

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  WB analysis of HepG2 using 16675-1-AP

  WB result of AMFR antibody (16675-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AMFR transfected HepG2 cells.

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  WB analysis of HepG2 using 16675-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16675-1-AP (AMFR antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 16675-1-AP

  HepG2 lysates were subjected to SDS PAGE followed by western blot with 16675-1-AP (AMFR antibody) at dilution of 1:4000 incubated at 37℃ and 100℃ for 1.5 hours.

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  WB analysis of MCF-7 using 16675-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 16675-1-AP (AMFR antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 16675-1-AP

  IP result of anti-AMFR (IP:16675-1-AP, 4ug; Detection:16675-1-AP 1:300) with MCF-7 cells lysate 2400 ug.

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  IHC staining of mouse ovary using 16675-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 16675-1-AP (AMFR/GP78 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse ovary using 16675-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 16675-1-AP (AMFR/GP78 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 16675-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16675-1-AP (AMFR antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 16675-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16675-1-AP (AMFR antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 16675-1-AP

  Immunofluorescent analysis of HepG2 cells, using AMFR antibody 16675-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: HepG2 cells, 37°C incubated HepG2 cells, MCF-7 cells
• Positive IP detected in: MCF-7 cells
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 8 publications below
• WB: See 21 publications below
• IHC: See 1 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

16675-1-AP targets AMFR/GP78 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AMFR/GP78 fusion protein Ag10178
• Full Name: autocrine motility factor receptor
• Calculated molecular weight: 643 aa, 73 kDa
• Observed molecular weight: 73 kDa
• GenBank accession number: BC069197
• Gene symbol: AMFR
• Gene ID (NCBI): 267
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AMFR(Autocrine motility factor receptor) is also named as RNF45,gp78.It is the receptor of Autocrine motility factor(AMF),which is a protein secreted by tumor cells that stimulates tumor motility. AMFR has a potential N-glycosylation site, and several potential O-glycosylation sites(PMID:10456327).In normal fibroblasts,the expression of AMFR appeared to be regulated by cell-cell contact(PMID:7626106).

Publications

Species	Application	Title
mouse	WB	Cell; BOK Is a Non-canonical BCL-2 Family Effector of Apoptosis Regulated by ER-Associated Degradation.; Authors - Fabien Llambi ; KD Validated
human, mouse	WB, IF	Immunity; The E3 Ubiquitin Ligase AMFR and INSIG1 Bridge the Activation of TBK1 Kinase by Modifying the Adaptor STING.; Authors - Qiang Wang ; KD Validated
human	IF	PLoS Biol; Reticulon and CLIMP-63 regulate nanodomain organization of peripheral ER tubules.; Authors - Guang Gao
human	WB	EMBO Rep; MARCH6 and TRC8 facilitate the quality control of cytosolic and tail-anchored proteins.; Authors - Sandra Stefanovic-Barrett ; KO Validated
human	WB	Elife; Aberrant corticosteroid metabolism in tumor cells enables GR takeover in enzalutamide resistant prostate cancer.; Authors - Jianneng Li ; KD Validated
human	WB	Elife; The sterol-responsive RNF145 E3 ubiquitin ligase mediates the degradation of HMG-CoA reductase together with gp78 and Hrd1.; Authors - Sam A Menzies