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AMFR/GP78 Polyclonal antibody
AMFR/GP78 Polyclonal Antibody for IF, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 16675-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HepG2 cells, 37°C incubated HepG2 cells, MCF-7 cells |
Positive IP detected in | MCF-7 cells |
Positive IF detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB |
Immunofluorescence (IF) | IF : 1:10-1:100 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 8 publications below |
WB | See 21 publications below |
IHC | See 1 publications below |
IF | See 4 publications below |
IP | See 1 publications below |
CoIP | See 1 publications below |
Product Information
16675-1-AP targets AMFR/GP78 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | AMFR/GP78 fusion protein Ag10178 |
Full Name | autocrine motility factor receptor |
Calculated molecular weight | 643 aa, 73 kDa |
Observed molecular weight | 73 kDa |
GenBank accession number | BC069197 |
Gene symbol | AMFR |
Gene ID (NCBI) | 267 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
AMFR(Autocrine motility factor receptor) is also named as RNF45,gp78.It is the receptor of Autocrine motility factor(AMF),which is a protein secreted by tumor cells that stimulates tumor motility. AMFR has a potential N-glycosylation site, and several potential O-glycosylation sites(PMID:10456327).In normal fibroblasts,the expression of AMFR appeared to be regulated by cell-cell contact(PMID:7626106).
Protocols
Product Specific Protocols | |
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WB protocol for AMFR/GP78 antibody 16675-1-AP | Download protocol |
IHC protocol for AMFR/GP78 antibody 16675-1-AP | Download protocol |
IF protocol for AMFR/GP78 antibody 16675-1-AP | Download protocol |
IP protocol for AMFR/GP78 antibody 16675-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell BOK Is a Non-canonical BCL-2 Family Effector of Apoptosis Regulated by ER-Associated Degradation.
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Immunity The E3 Ubiquitin Ligase AMFR and INSIG1 Bridge the Activation of TBK1 Kinase by Modifying the Adaptor STING.
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PLoS Biol Reticulon and CLIMP-63 regulate nanodomain organization of peripheral ER tubules. | ||
EMBO Rep MARCH6 and TRC8 facilitate the quality control of cytosolic and tail-anchored proteins.
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Elife Aberrant corticosteroid metabolism in tumor cells enables GR takeover in enzalutamide resistant prostate cancer.
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Elife The sterol-responsive RNF145 E3 ubiquitin ligase mediates the degradation of HMG-CoA reductase together with gp78 and Hrd1. |