AMBRA1 Polyclonal antibody

• Featured Product
• KD/KO Validated

AMBRA1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

15

Cat no : 13762-1-AP

Synonyms

WDR94, KIAA1736, DDB1- and CUL4-associated factor 3, DCAF3, Activating molecule in BECN1-regulated autophagy protein 1

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Validation Data Gallery

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  WB analysis of MCF-7 using 13762-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 13762-1-AP (AMBRA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-1080 using 13762-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 13762-1-AP (AMBRA1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 13762-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13762-1-AP (AMBRA1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 13762-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13762-1-AP (AMBRA1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 13762-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13762-1-AP (AMBRA1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 13762-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 13762-1-AP (AMBRA1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 13762-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13762-1-AP (AMBRA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 13762-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13762-1-AP (AMBRA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 13762-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using AMBRA1 antibody (13762-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of MCF-7 using 13762-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using AMBRA1 antibody (13762-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: MCF-7 cells, human brain tissue, HT-1080 cells, HepG2 cells, mouse brain tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 15 publications below
• IHC: See 4 publications below
• IF: See 2 publications below
• IP: See 3 publications below

Product Information

13762-1-AP targets AMBRA1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AMBRA1 fusion protein Ag4735
• Full Name: autophagy/beclin-1 regulator 1
• Calculated Molecular Weight: 1208 aa, 133 kDa
• Observed Molecular Weight: 130-135 kDa
• GenBank Accession Number: BC045609
• Gene Symbol: AMBRA1
• Gene ID (NCBI): 55626
• RRID: AB_10642007
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AMBRA1 (Activating molecule in BECN1-regulated autophagy protein 1), also known as WDR94 or KIAA1736, is a 1,298 amino acid protein that contains three WD repeats. AMBRA1 regulates autophagy and development of the nervous system. Involved in autophagy in controlling protein turnover during neuronal development, and in regulating normal cell survival and proliferation. This antibody works well in WB, IHC and IF application.

Publications

Species	Application	Title
human	WB,IHC	Nature; CRL4AMBRA1 is a master regulator of D-type cyclins.; Authors - Daniele Simoneschi ; KO Validated
human	WB	Pharmacol Res; miR-103a-3p regulates mitophagy in Parkinson's disease through Parkin/Ambra1 signaling.; Authors - Junjun Zhou
mouse	WB	Neurobiol Dis; Neuron-astrocyte transmitophagy is altered in Alzheimer's disease.; Authors - Riikka Lampinen
human	WB,IHC	Brain Pathol; AMBRA1, a novel α-synuclein-binding protein, is implicated in the pathogenesis of multiple system atrophy.; Authors - Yasuo Miki ; KD Validated
human	WB	Front Cell Dev Biol; Autophagy-Related Genes and Long Noncoding RNAs Signatures as Predictive Biomarkers for Osteosarcoma Survival.; Authors - Jian Zhang
human	WB	Int J Biochem Cell Biol; Selenoprotein S attenuates high glucose and/or ox-LDL-induced endothelium injury by regulating Akt/mTOR signaling and autophagy.; Authors - Zinan Wang