AMBRA1 Polyclonal antibody

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• KD/KO Validated

AMBRA1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 13762-1-AP Copied!

Publications (29)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

Activating molecule in BECN1-regulated autophagy protein 1, DCAF3, DDB1- and CUL4-associated factor 3, KIAA1736, WDR94

• 

  WB analysis of MCF-7 using 13762-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 13762-1-AP (AMBRA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 13762-1-AP

  U2OS cells were subjected to SDS PAGE followed by western blot with 13762-1-AP (AMBRA1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human brain using 13762-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13762-1-AP (AMBRA1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of MCF-7 using 13762-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 13762-1-AP (AMBRA1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HT-1080 using 13762-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 13762-1-AP (AMBRA1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 13762-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13762-1-AP (AMBRA1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse brain using 13762-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13762-1-AP (AMBRA1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 13762-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13762-1-AP (AMBRA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 13762-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13762-1-AP (AMBRA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 13762-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using AMBRA1 antibody (13762-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of MCF-7 using 13762-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using AMBRA1 antibody (13762-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: MCF-7 cells, HepG2 cells, HT-1080 cells, human brain tissue, mouse brain tissue, U2OS cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13762-1-AP targets AMBRA1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4735

  Product name: Recombinant human AMBRA1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 906-1208 aa of BC045609

  Sequence: NVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEALNSGVEYYWDQLNETVFTVHSNSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPATSVTSQGTETLALQLQNAETQTEREVPEPGTAASGPGEGEGSEYGASGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTEPGAAHTSSPQPSTSRGLLPEAGQLAERGLSPRTASWDQPGTPGREPTQPTLPSSSPVPIPVSLPSAEGPTVHCELTNNNHLLDGGSSRGDAAGPRGEPRNR

• Full Name: autophagy/beclin-1 regulator 1
• Calculated Molecular Weight: 1208 aa, 133 kDa
• Observed Molecular Weight: 110kDa, 130-135 kDa
• GenBank Accession Number: BC045609
• Gene Symbol: AMBRA1
• Gene ID (NCBI): 55626
• RRID: AB_10642007
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9C0C7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AMBRA1 (Activating molecule in BECN1-regulated autophagy protein 1), also known as WDR94 or KIAA1736, is a 1,298 amino acid protein that contains three WD repeats. AMBRA1 regulates autophagy and development of the nervous system. Involved in autophagy in controlling protein turnover during neuronal development, and in regulating normal cell survival and proliferation. This antibody works well in WB, IHC and IF application. The full length of Ambra1 is about 140 kDa, with the C-terminal fragment measuring approximately 100 kDa and the N-terminal fragment measuring approximately 50 kDa (PMID: 22441670).

Publications

What published studies show

AMBRA1 antibody (Cat# 13762-1-AP) has 29 citations across journals including Nature, Nature Communications, Advanced Science, Cell Death & Differentiation, Developmental Cell, eLife, and Nature Immunology. Research fields span autophagy/mitophagy regulation, cancer biology (lung, breast, lymphoma, melanoma), neurodegeneration (Parkinson's, Alzheimer's), inflammation, cell cycle control, and immune signaling.

Automatically generated from the 29 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IHC	Nature; CRL4AMBRA1 is a master regulator of D-type cyclins.; Authors - Daniele Simoneschi ; KO Validated
human	WB	Nat Commun; F-box protein FBXO32 ubiquitinates and stabilizes D-type cyclins to drive cancer progression; Authors - Feng Li
mouse,human	WB,IP,IHC	Adv Sci (Weinh); Redox-Induced Stabilization of AMBRA1 by USP7 Promotes Intestinal Oxidative Stress and Colitis Through Antagonizing DUB3-Mediated NRF2 Deubiquitination; Authors - Weimin Xu ; KD Validated
human	WB,IP,IHC	Adv Sci (Weinh); CDK4/6 Inhibitors Impede Chemoresistance and Inhibit Tumor Growth of Small Cell Lung Cancer; Authors - Yang Wen ; KD Validated
human	WB,IP,IHC	Cell Death Differ; AMBRA1 promotes intestinal inflammation by antagonizing PP4R1/PP4c mediated IKK dephosphorylation in an autophagy-independent manner; Authors - Weimin Xu
human	WB	Pharmacol Res; miR-103a-3p regulates mitophagy in Parkinson's disease through Parkin/Ambra1 signaling.; Authors - Junjun Zhou