Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, A431 cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:300-1:1200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16690-1-AP targets ALY in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10044 Product name: Recombinant human ALY protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 5-257 aa of BC052302 Sequence: MDMSLDDIIKLNRSQRGGRGGGRGRGRAGSQGGRGGGAQAAARVNRGGGPIRNRPAIARGAAGGGGRNRPAPYSRPKQLPDKWQHDLFDSGFGGGAGVETGGKLLVSNLDFGVSDADIQELFAEFGTLKKAAVHYDRSGRSLGTADVHFERKADALKAMKQYNGVPLDGRPMNIQLVTSQIDAQRRPAQSVNRGGMTRNRGAGGFGGGGGTRRGTRGGARGRGRGAGRNSKQQLSAEELDAQLDAYNARMDTS Predict reactive species |
| Full Name | THO complex 4 |
| Calculated Molecular Weight | 257 aa, 27 kDa |
| Observed Molecular Weight | 27-30 kDa |
| GenBank Accession Number | BC052302 |
| Gene Symbol | ALY |
| Gene ID (NCBI) | 10189 |
| RRID | AB_2878300 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86V81 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ALY antibody 16690-1-AP | Download protocol |
| IHC protocol for ALY antibody 16690-1-AP | Download protocol |
| WB protocol for ALY antibody 16690-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ALY Polyclonal antibody (Cat# 16690-1-AP) has 21 citations across journals including Nature, Cell, Cell Metabolism, EMBO Journal, Cell Death & Disease, and PLoS Pathogens. Research fields span RNA m5C modification, cancer biology (ovarian, breast, prostate, colorectal, esophageal, and bladder cancers), viral replication, ferroptosis, sepsis, liver disease, neuroblastoma, and corneal neovascularization. Applications cited include WB, IHC, IF, IP, and RIP in human, mouse, rat, and pig samples.
| Species | Application | Title |
|---|---|---|
Cell Metab NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis. | ||
Exp Mol Med RNA m5C modification upregulates E2F1 expression in a manner dependent on YBX1 phase separation and promotes tumor progression in ovarian cancer | ||
Cell Death Dis Multi-omics analysis reveals that ALYREF-mediated m(5)C modification promotes platinum resistance in ovarian cancer via the NSUN2/ALYREF/LGR4 axis.
| ||
Cell Death Dis Epigenetic addition of m5C to HBV transcripts promotes viral replication and evasion of innate antiviral responses |
Reviews
What customers say
The single review for this product is from a user who tested it by Western Blot on HeLa cell extracts at a 1:1000 dilution with overnight incubation at 4°C. They rated it 5 out of 5 stars and noted that while the band was weak, it was specific for ALYREF/THOC4. Overall, the reviewer found the antibody to be specific despite the modest signal intensity.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Roy (Verified Customer) (06-15-2024) | Weak band on WB from HeLa cells extracts but specific for ALYREF/THOC4 (1/1000 dilution, Overnight incubation at 4°C)
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