ALPL Polyclonal antibody, PBS Only

ALPL Polyclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

Cat no : 11187-1-PBS

Synonyms

Alkaline phosphatase, AKP2, AP, Alkaline phosphatase, tissue-nonspecific isozyme, Alkaline phosphatase tissue nonspecific isozyme

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Validation Data Gallery

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  WB analysis using 11187-1-AP (same clone as 11187-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 11187-1-AP (ALPL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11187-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 11187-1-AP (same clone as 11187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11187-1-AP (ALPL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11187-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 11187-1-AP (same clone as 11187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11187-1-AP (ALPL antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11187-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 11187-1-AP (same clone as 11187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11187-1-AP (ALPL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11187-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 11187-1-AP (same clone as 11187-1-PBS)

  Immunofluorescent analysis of HEK-293 cells using 11187-1-AP (ALPL antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11187-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 11187-1-AP (same clone as 11187-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human ALPL (11187-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 11187-1-PBS in a different storage buffer formulation.

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Product Information

11187-1-PBS targets ALPL in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ALPL fusion protein Ag1664
• Full Name: alkaline phosphatase, liver/bone/kidney
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 80 kDa
• GenBank Accession Number: BC021289
• Gene Symbol: ALPL
• Gene ID (NCBI): 249
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. ALPL(Alkaline phosphatase, tissue-nonspecific isozyme) is also named as AP-TNAP, TNSALP and belongs to the alkaline phosphatase family. It can exsit as a homodimer with the molecular weight of 140 kDa(PMID:18724009). ALPL was synthesized as a 66-kDa endo-b-N-acetylglucos-aminidase H (Endo H)-sensitive form, and processed to an 80-kDa mature form, which is anchored to the plasma membrane via glycosylphosphatidylinositol (GPI)(PMID:10839996).This antibody can bind the four mentioned alkaline phosphatases.