ALPK1 Polyclonal antibody

ALPK1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 19107-1-AP

Synonyms

8430410J10Rik, alpha kinase 1, Alpha protein kinase 1, ALPK, ALPK1, Chromosome 4 kinase, KIAA1527, LAK

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Validation Data Gallery

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  WB analysis using 19107-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19107-1-AP (ALPK1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 19107-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19107-1-AP (ALPK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: COLO 320 cells, HEK-293 cells, HepG2 cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 3 publications below
• IF: See 4 publications below

Product Information

19107-1-AP targets ALPK1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ALPK1 fusion protein Ag5320
• Full Name: alpha-kinase 1
• Calculated Molecular Weight: 139 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: BC060780
• Gene Symbol: ALPK1
• Gene ID (NCBI): 80216
• RRID: AB_10638642
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Alpha-protein kinase 1, also known as alpha-kinase 1 (ALPK1), is associated with chronic kidney disease (CKD), myocardial infarction, gout and type 2 diabetes mellitus (DM). ALPK1 mutant has been reported to cause severe defects in motor co‐ordination in mice. ALPK1 enhances the expression of proinflammatory cytokines including IL-1β, IL-8 and TGF-β1 in cultured human THP1 and human embryonic kidney 293 (HEK293) cells (PMID: 31557402, PMID: 26275947). ALPK1 short isoform (108 kDa) was highly expressed in brain, spinal cord, heart, lung, spleen, thymus, small intestine, skin and testis, while detectable inskeletal muscle and kidney. ALPK1 large isoform (130 kDa) appeared in heart, lung, thymus and skin (PMID: 21208416).

Publications

Species	Application	Title
mouse	WB	Front Immunol; Alpk1 Sensitizes Pancreatic Beta Cells to Cytokine-Induced Apoptosis via Upregulating TNF-α Signaling Pathway.; Authors - Fei Ding
human,mouse	WB,IHC	J Dent Res; ALPK1 Aggravates TMJOA Cartilage Degradation via NF-κB and ERK1/2 Signaling.; Authors - X Liu
mouse	IHC	J Bone Miner Res; ALPK1 Accelerates the Pathogenesis of Osteoarthritis by Activating NLRP3 Signaling; Authors - Xin Liu
mouse	WB	Int Immunopharmacol; Neuroprotective effect of alpha-kinase 1 knockdown against cerebral ischemia through inhibition of the NF-κB pathway and neuroinflammation; Authors - Jing-Ying Li
mouse	IF	Mol Neurobiol; ALPK1 Expressed in IB4-Positive Neurons of Mice Trigeminal Ganglions Promotes MIA-Induced TMJ pain; Authors - Taomin Zhu
human,mouse	IHC,IF	Int Immunopharmacol; Pyroptosis of chondrocytes activated by synovial inflammation accelerates TMJ osteoarthritis cartilage degeneration via ROS/NLRP3 signaling; Authors - Xin Liu