ALDOA Polyclonal antibody

• Featured Product
• KD/KO Validated

ALDOA Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Conjugate

Unconjugated

Publications

54

Reviews (1)

Cat no : 11217-1-AP

Synonyms

Muscle-type aldolase, Lung cancer antigen NY-LU-1, Fructose-bisphosphate aldolase A, EC:4.1.2.13, Aldolase A

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Validation Data Gallery

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  WB analysis using 11217-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11217-1-AP (ALDOA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11217-1-AP

  WB result of ALDOA antibody (11217-1-AP; 1:240000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ALDOA transfected HeLa cells.

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  WB analysis using 11217-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11217-1-AP (ALDOA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11217-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11217-1-AP (ALDOA antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11217-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11217-1-AP (ALDOA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 11217-1-AP

  IP result of anti-ALDOA (IP:11217-1-AP, 3ug; Detection:11217-1-AP 1:1000) with HepG2 cells lysate 6000ug.

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  IHC staining of human colon cancer using 11217-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11217-1-AP (ALDOA Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 11217-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11217-1-AP (ALDOA Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 11217-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11217-1-AP (ALDOA antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 11217-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11217-1-AP (ALDOA antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 11217-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11217-1-AP (ALDOA antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 11217-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11217-1-AP (ALDOA antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 11217-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 11217-1-AP (ALDOA antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 11217-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 11217-1-AP (ALDOA antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 11217-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ALDOA antibody (11217-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 11217-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ALDOA antibody (11217-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, A549 cells, Jurkat cells, mouse liver tissue, rat liver tissue, HepG2 cells, MCF-7 cells, NIH/3T3 cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human colon cancer tissue, human lung cancer tissue, human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 48 publications below
• IHC: See 8 publications below
• IF: See 9 publications below
• IP: See 4 publications below
• CoIP: See 2 publications below
• RIP: See 1 publications below

Product Information

11217-1-AP targets ALDOA in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, sheep
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ALDOA fusion protein Ag1726
• Full Name: aldolase A, fructose-bisphosphate
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC016800
• Gene Symbol: ALDOA
• Gene ID (NCBI): 226
• RRID: AB_2224626
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ALDOA(Fructose-bisphosphate aldolase A) is also named as lung cancer antigen NY-LU-1,muscle-type aldolase,ALDA and belongs to the class I fructose-bisphosphate aldolase family.The ALDOA gene encodes a homotetrameric protein(PMID:14615364).It is found in skeletal muscle and red blood cells, and functions in glycolysis, promoting the reversible cleavage of FBP to triose phosphate, G-3-P and DHAP. ALDOA is upregulated in various cancers, including human lung squamous and renal cell carcinomas(PMID:19937139).

Publications

Species	Application	Title
human	WB	Cancer Cell; Transcriptional Regulation of the Warburg Effect in Cancer by SIX1.; Authors - Ling Li
mouse	WB	Nat Neurosci; Metabolic lactate production coordinates vasculature development and progenitor behavior in the developing mouse neocortex.; Authors - Xiaoxiang Dong
mouse	WB	Dev Cell; Spatiotemporal Analysis of a Glycolytic Activity Gradient Linked to Mouse Embryo Mesoderm Development.; Authors - Vinay Bulusu
human,mouse	WB	Int J Biol Sci; Chronic stress promotes colorectal cancer progression by enhancing glycolysis through β2-AR/CREB1 signal pathway; Authors - Yunfeng Guan
human	WB	Cell Death Dis; The ubiquitin-like protein UBTD1 promotes colorectal cancer progression by stabilizing c-Myc to upregulate glycolysis; Authors - Liqin Zhao
human	IF	Mol Ther; Single-Cell Transcriptome Analysis Reveals Intratumoral Heterogeneity in ccRCC, which Results in Different Clinical Outcomes.; Authors - Junyi Hu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

K (Verified Customer) (12-20-2020)

Worked well for IHC at 1:200 dilution

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: mouse liver tissues and rat liver