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ALDOA Polyclonal antibody
ALDOA Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (2)
Applications
WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA
Conjugate
Unconjugated
Cat no : 11217-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, HeLa cells, A549 cells, Jurkat cells, mouse liver tissue, rat liver tissue, HepG2 cells, MCF-7 cells, NIH/3T3 cells |
Positive IP detected in | HepG2 cells |
Positive IHC detected in | human colon cancer tissue, human lung cancer tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 5 publications below |
WB | See 46 publications below |
IHC | See 7 publications below |
IF | See 9 publications below |
IP | See 4 publications below |
CoIP | See 2 publications below |
RIP | See 1 publications below |
Product Information
11217-1-AP targets ALDOA in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, pig, sheep |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | ALDOA fusion protein Ag1726 |
Full Name | aldolase A, fructose-bisphosphate |
Calculated Molecular Weight | 39 kDa |
Observed Molecular Weight | 35-40 kDa |
GenBank Accession Number | BC016800 |
Gene Symbol | ALDOA |
Gene ID (NCBI) | 226 |
RRID | AB_2224626 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ALDOA(Fructose-bisphosphate aldolase A) is also named as lung cancer antigen NY-LU-1,muscle-type aldolase,ALDA and belongs to the class I fructose-bisphosphate aldolase family.The ALDOA gene encodes a homotetrameric protein(PMID:14615364).It is found in skeletal muscle and red blood cells, and functions in glycolysis, promoting the reversible cleavage of FBP to triose phosphate, G-3-P and DHAP. ALDOA is upregulated in various cancers, including human lung squamous and renal cell carcinomas(PMID:19937139).
Protocols
Product Specific Protocols | |
---|---|
WB protocol for ALDOA antibody 11217-1-AP | Download protocol |
IHC protocol for ALDOA antibody 11217-1-AP | Download protocol |
IF protocol for ALDOA antibody 11217-1-AP | Download protocol |
IP protocol for ALDOA antibody 11217-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Neurosci Metabolic lactate production coordinates vasculature development and progenitor behavior in the developing mouse neocortex. | ||
Dev Cell Spatiotemporal Analysis of a Glycolytic Activity Gradient Linked to Mouse Embryo Mesoderm Development. | ||
Int J Biol Sci Chronic stress promotes colorectal cancer progression by enhancing glycolysis through β2-AR/CREB1 signal pathway | ||
Mol Ther Single-Cell Transcriptome Analysis Reveals Intratumoral Heterogeneity in ccRCC, which Results in Different Clinical Outcomes. | ||
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH K (Verified Customer) (12-20-2020) | Worked well for IHC at 1:200 dilution
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