ALDH8A1 Polyclonal antibody

ALDH8A1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 21602-1-AP

Synonyms

EC:1.2.1.32, ALDH8A 1, ALDH12, 2-aminomuconic semialdehyde dehydrogenase

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Validation Data Gallery

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  WB analysis using 21602-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21602-1-AP (ALDH8A1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 21602-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 21602-1-AP (ALDH8A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 21602-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 21602-1-AP (ALDH8A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 21602-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21602-1-AP (ALDH8A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human hepatocirrhosis using 21602-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 21602-1-AP (ALDH8A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human hepatocirrhosis using 21602-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 21602-1-AP (ALDH8A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 21602-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 21602-1-AP (ALDH8A1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: L02 cells, mouse kidney tissue, mouse liver tissue, rat liver tissue
• Positive IHC detected in: human hepatocirrhosis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21602-1-AP targets ALDH8A1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ALDH8A1 fusion protein Ag16214
• Full Name: aldehyde dehydrogenase 8 family, member A1
• Calculated Molecular Weight: 487 aa, 53 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC113862
• Gene Symbol: ALDH8A1
• Gene ID (NCBI): 64577
• RRID: AB_10733887
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.