Validation Data Gallery
|Positive WB detected in||mouse liver tissue, mouse liver, rat liver|
|Positive IP detected in||mouse liver tissue|
|Positive IHC detected in||human gliomas tissue, mouse brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse brain tissue|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
17390-1-AP targets ALDH1L1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ALDH1L1 fusion protein Ag11285|
|Full Name||aldehyde dehydrogenase 1 family, member L1|
|Calculated molecular weight||99 kDa|
|Observed molecular weight||100 kDa|
|GenBank accession number||BC027241|
|Gene ID (NCBI)||10840|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Aldehyde dehydrogenase 1 family member L1 (ALDH1L1), belongs to the aldehyde dehydrogenase family of proteins and is responsible for formate oxidation in vivo. Loss of ALDH1L1 is associated with decreased apoptosis, increased cell motility, and cancer progression. ALDH1L1 catalyzes the conversion of 10-formyltetrahydrofolate, nicotinamide adenine dinucleotide phosphate (NADP+), and water to tetrahydrofolate, NADPH, and carbon dioxide. ALDH1L1 has some isoforms with MW of 99, 88 and 55 kDa.
|Product Specific Protocols|
|WB protocol for ALDH1L1 antibody 17390-1-AP||Download protocol|
|IHC protocol for ALDH1L1 antibody 17390-1-AP||Download protocol|
|IF protocol for ALDH1L1 antibody 17390-1-AP||Download protocol|
|IP protocol for ALDH1L1 antibody 17390-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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Juan (Verified Customer) (01-11-2022)