|Positive WB detected in
|HepG2 cells, mouse liver tissue, rat liver tissue
|Positive IHC detected in
|human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Western Blot (WB)
|WB : 1:500-1:2000
|IHC : 1:50-1:500
|IF : 1:20-1:200
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
The immunogen of 25167-1-AP is ALDH1A3 Fusion Protein expressed in E. coli.
|human, mouse, rat
|human, mouse, goat
|Host / Isotype
|Rabbit / IgG
|ALDH1A3 fusion protein Ag18605
|aldehyde dehydrogenase 1 family, member A3
|Calculated molecular weight
|512 aa, 56 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Aldehyde dehydrogenase 1 family member A3 (ALDH1A3), is an aldehyde dehydrogenase enzyme that uses retinal as a substrate either in a free or a cellular retinol-binding protein form. ALDH1A3 plays a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation.ALDH1A3 is calculated to be 56 kDa.
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Azita (Verified Customer) (06-16-2021)
Immunocytochemistry labelling of (4% PFA) fixed mice spinal cord tissue by ALDH1A3 antibody at dilution of 1:100.