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ALDH1A3 Polyclonal antibody

ALDH1A3 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (1)





Cat no : 25167-1-AP


Aldehyde dehydrogenase 6, ALDH1A3, ALDH1A6, ALDH6, RALDH 3, RALDH3, Retinaldehyde dehydrogenase 3

Tested Applications

Positive WB detected inHepG2 cells, mouse liver tissue, rat liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 25167-1-AP is ALDH1A3 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, goat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ALDH1A3 fusion protein Ag18605
Full Name aldehyde dehydrogenase 1 family, member A3
Calculated molecular weight 512 aa, 56 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC069274
Gene symbol ALDH1A3
Gene ID (NCBI) 220
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Aldehyde dehydrogenase 1 family member A3 (ALDH1A3), is an aldehyde dehydrogenase enzyme that uses retinal as a substrate either in a free or a cellular retinol-binding protein form. ALDH1A3 plays a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation.ALDH1A3 is calculated to be 56 kDa.


Product Specific Protocols
WB protocol for ALDH1A3 antibody 25167-1-APDownload protocol
IHC protocol for ALDH1A3 antibody 25167-1-APDownload protocol
IF protocol for ALDH1A3 antibody 25167-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Azita (Verified Customer) (06-16-2021)

Immunocytochemistry labelling of (4% PFA) fixed mice spinal cord tissue by ALDH1A3 antibody at dilution of 1:100.

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: mice lumbar spinal cord tissue (transverse section 10 µm)