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ALDH1A3 Polyclonal antibody

ALDH1A3 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 25167-1-AP

Synonyms

Aldehyde dehydrogenase 6, ALDH1A3, ALDH1A6, ALDH6, RALDH 3, RALDH3, Retinaldehyde dehydrogenase 3



Tested Applications

Positive WB detected inHepG2 cells, mouse liver tissue, rat liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 25167-1-AP is ALDH1A3 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, goat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ALDH1A3 fusion protein Ag18605
Full Name aldehyde dehydrogenase 1 family, member A3
Calculated molecular weight 512 aa, 56 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC069274
Gene symbol ALDH1A3
Gene ID (NCBI) 220
RRIDAB_2879937
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Aldehyde dehydrogenase 1 family member A3 (ALDH1A3), is an aldehyde dehydrogenase enzyme that uses retinal as a substrate either in a free or a cellular retinol-binding protein form. ALDH1A3 plays a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation.ALDH1A3 is calculated to be 56 kDa.

Protocols

Product Specific Protocols
WB protocol for ALDH1A3 antibody 25167-1-APDownload protocol
IHC protocol for ALDH1A3 antibody 25167-1-APDownload protocol
IF protocol for ALDH1A3 antibody 25167-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nature

A human embryonic limb cell atlas resolved in space and time

Authors - Bao Zhang
humanWB

Nat Commun

Decoding non-canonical mRNA decay by the endoplasmic-reticulum stress sensor IRE1α

Authors - Adrien Le Thomas
humanWB

Cell Res

Excessive UBE3A dosage impairs retinoic acid signaling and synaptic plasticity in autism spectrum disorders.

Authors - Xingxing Xu
humanWB

Cell Chem Biol

Lipid-derived electrophiles mediate the effects of chemotherapeutic topoisomerase I poisons.

Authors - Amy Flor
mouseWB

Mol Metab

Autotaxin signaling facilitates β cell dedifferentiation and dysfunction induced by Sirtuin 3 deficiency.

Authors - Huanyi Cao
humanWB

Free Radic Biol Med

Flavonoid 4,4'-dimethoxychalcone suppresses cell proliferation via dehydrogenase inhibition and oxidative stress aggravation.

Authors - Changmei Yang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Azita (Verified Customer) (06-16-2021)

Immunocytochemistry labelling of (4% PFA) fixed mice spinal cord tissue by ALDH1A3 antibody at dilution of 1:100.

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: mice lumbar spinal cord tissue (transverse section 10 µm)