ALDH1A1-specific Polyclonal antibody

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• KD/KO Validated

ALDH1A1-specific Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 22109-1-AP

Synonyms

ALDH1A1, ALDH1A 1, ALDH1 A1, ALDH 1A1, Aldehyde dehydrogenase 1A1

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Validation Data Gallery

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  WB analysis of human liver using 22109-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 22109-1-AP (ALDH1A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 22109-1-AP

  WB result of ALDH1A1 antibody (22109-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ALDH1A1 transfected K-562 cells.

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  WB analysis using 22109-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22109-1-AP (ALDH1A1-specific antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 22109-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22109-1-AP (ALDH1A1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 22109-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22109-1-AP (ALDH1A1 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of A549 using 22109-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using ALDH1A1-specific antibody (22109-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: A549 cells, human liver tissue, K-562 cells, HepG2 cells, mouse liver tissue
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

22109-1-AP targets ALDH1A1-specific in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ALDH1A1-specific fusion protein Ag17358
• Full Name: aldehyde dehydrogenase 1 family, member A1
• Calculated Molecular Weight: 501 aa, 55 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC001505
• Gene Symbol: ALDH1A1
• Gene ID (NCBI): 216
• RRID: AB_10949189
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ALDH1A1(Aldehyde dehydrogenase family 1 member A1 ), also named as ALDC, ALDH1 and PUMB1, belongs to the aldehyde dehydrogenase family. The ALDH1A1 gene encodes a liver cytosolic isoform of acetaldehyde dehydrogenase, an enzyme involved in the major pathway of alcohol metabolism after alcohol dehydrogenase. ALDH1A1 plays a critical role in protection against oxidative stress-induced cytotoxicity in lens epithelial cells(PMID:19296407). And it is important for multiple biological activities including drug resistance, cell differentiation, and oxidative stress response(PMID:19025616). As a novel cancer stem cell marker, ALDH1A1 can be used for tumors whose corresponding normal tissues express ALDH1 in relatively restricted or limited levels such as breast, lung, ovarian or colon cancer(PMID: 20422001). This antibody can also recognize some other membranes of the aldehyde dehydrogenase family. This antibody is specific to ALDH1A1.

Publications

Species	Application	Title
human,mouse	WB	Cancer Discov; Transcriptional Silencing of ALDH2 Confers a Dependency on Fanconi Anemia Proteins in Acute Myeloid Leukemia.; Authors - Zhaolin Yang
human	IHC	Cell Rep Med; Distinctive multicellular immunosuppressive hubs confer different intervention strategies for left- and right-sided colon cancers; Authors - Bingxin Liu
human	WB	Theranostics; PMID: 28839463; Authors - Junlong Zhuang
human	IP	Commun Biol; PRMT3 interacts with ALDH1A1 and regulates gene-expression by inhibiting retinoic acid signaling.; Authors - Mamta Verma
human	WB	Int J Oncol; Parthenolide inhibits the tumor characteristics of renal cell carcinoma.; Authors - Dandan Liu
human	WB	Neoplasma; Long noncoding RNA ASAP1-IT1 promotes cancer stemness and predicts a poor prognosis in patients with bladder cancer.; Authors - L Yang