ALDH1A1 Polyclonal antibody

• Featured Product
• KD/KO Validated

ALDH1A1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

130

Reviews (6)

Cat no : 15910-1-AP

Synonyms

ALDH1A 1, ALDH1 A1, ALDH 1A1, Aldehyde dehydrogenase 1A1, ALDC

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Validation Data Gallery

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  WB analysis using 15910-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15910-1-AP (ALDH1A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15910-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15910-1-AP (ALDH1A1 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15910-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15910-1-AP (ALDH1A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 15910-1-AP

  IP result of anti-ALDH1A1 (IP:15910-1-AP, 4ug; Detection:15910-1-AP 1:20000) with mouse liver tissue lysate 2200 ug.

• 

  IHC staining of mouse testis using 15910-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15910-1-AP (ALDH1A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 15910-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15910-1-AP (ALDH1A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 15910-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 15910-1-AP (ALDH1A1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 15910-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15910-1-AP (ALDH1A1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 15910-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15910-1-AP (ALDH1A1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of mouse colon using 15910-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using 15910-1-AP (ALDH1A1 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse colon using 15910-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using 15910-1-AP (ALDH1A1 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse testis using 15910-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using 15910-1-AP (ALDH1A1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A549 using 15910-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using ALDH1A1 antibody (15910-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 15910-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human ALDH1A1 (15910-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: K-562 cells, mouse liver tissue, rat liver tissue, mouse brain tissue, mouse kidney tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: mouse testis tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse colon tissue, mouse testis tissue
• Positive IF/ICC detected in: A549 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 110 publications below
• IHC: See 25 publications below
• IF: See 15 publications below
• ELISA: See 2 publications below
• CoIP: See 1 publications below

Product Information

15910-1-AP targets ALDH1A1 in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ALDH1A1 fusion protein Ag8665
• Full Name: aldehyde dehydrogenase 1 family, member A1
• Calculated Molecular Weight: 501 aa, 55 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC001505
• Gene Symbol: ALDH1A1
• Gene ID (NCBI): 216
• RRID: AB_2305276
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ALDH1A1(Aldehyde dehydrogenase family 1 member A1 ), also named as ALDC, ALDH1 and PUMB1, belongs to the aldehyde dehydrogenase family. The ALDH1A1 gene encodes a liver cytosolic isoform of acetaldehyde dehydrogenase, an enzyme involved in the major pathway of alcohol metabolism after alcohol dehydrogenase. ALDH1A1 plays a critical role in protection against oxidative stress-induced cytotoxicity in lens epithelial cells(PMID:19296407). And it is important for multiple biological activities including drug resistance, cell differentiation, and oxidative stress response(PMID:19025616). As a novel cancer stem cell marker, ALDH1A1 can be used for tumors whose corresponding normal tissues express ALDH1 in relatively restricted or limited levels such as breast, lung, ovarian or colon cancer(PMID: 20422001). This antibody can also recognize some other membranes of the aldehyde dehydrogenase family.

Publications

Species	Application	Title
human	WB	Cell Res; Excessive UBE3A dosage impairs retinoic acid signaling and synaptic plasticity in autism spectrum disorders.; Authors - Xingxing Xu
mouse	WB	Mol Cell; Maternal Aldehyde Elimination during Pregnancy Preserves the Fetal Genome.; Authors - Nina Oberbeck
human	WB,IHC,IF	Int J Oral Sci; LIMP-2 enhances cancer stem-like cell properties by promoting autophagy-induced GSK3β degradation in head and neck squamous cell carcinoma; Authors - Yuantong Liu
human	WB,IHC	Nat Commun; TSPAN8 promotes cancer cell stemness via activation of sonic Hedgehog signaling.; Authors - Rongxuan Zhu
human,mouse	WB,IHC,IF	J Pineal Res; Melatonin inhibits the stemness of head and neck squamous cell carcinoma by modulating HA synthesis via the FOSL1/HAS3 axis; Authors - Xinyue Luo
human	IF	EMBO J; RASSF1C oncogene elicits amoeboid invasion, cancer stemness, and extracellular vesicle release via a SRC/Rho axis.; Authors - Maria Laura Tognoli

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Shinford Z. (Verified Customer) (01-31-2024)

The antibody performs exceptionally well; the band on the membrane is remarkably distinct.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Cancer cell lines

ALDH1A1 Antibody Western Blot validation (1:1000 dilution) in Cancer cell lines (Cat no:15910-1-AP)

FH

Matthew (Verified Customer) (08-07-2023)

Antibody worked perfectly with overnight incubation at 4C. Diluted 1:1000 in TBST.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse mammary epithelial cell

FH

Udesh (Verified Customer) (02-24-2023)

Worked well at 1:1000 in 5% BSA O/N at 4 degrees

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: U2OS Osteosarcoma cells

ALDH1A1 Antibody Western Blot validation (1:1000 dilution) in U2OS Osteosarcoma cells (Cat no:15910-1-AP)

FH

Susmita (Verified Customer) (09-17-2020)

Antibody is working good for WB

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000 WB;

FH

Mario Armando (Verified Customer) (02-18-2020)

I used this ALDH1A1 antibody on different human and mouse tissues including adipose tissue, heart and skeletal muscle. It worked wonderfully even when using paraffin embedded sections in both human and mouse.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: OCT embedded frozen section of Human Adipose tissue

ALDH1A1 Antibody Immunofluorescence, validation (1:100 dilution) in OCT embedded frozen section of Human Adipose tissue (Cat no:15910-1-AP)

FH

Joleen (Verified Customer) (06-07-2019)

Prominent band correspond to ALDH1A1. Works well in western blot.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1k
• Cell Tissue Type: MDCK GII

ALDH1A1 Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:15910-1-AP)

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