PE Anti-Human ALCAM (3A6)

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  FC experiment of human PBMCs using PE-65223

  1X10^6 PHA-treated human PBMCs were surface stained with 5 ul PE Anti-Human ALCAM (PE-65223, Clone:3A6) (red) or unstained (blue). The black dashed line indicates PBMCs surface stained with 5 ul PE Anti-Human ALCAM. Cells were not fixed. Monocytes were gated.

ALCAM Monoclonal Antibody for FC

Cat No. PE-65223

Clone No.3A6

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Synonyms

ALCAM, CD166, CD166 antigen, FLJ38514, MEMD

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Tested Applications

• Positive FC detected in: PHA-treated human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in 100 μl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

PE-65223 targets ALCAM in FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Cultured human thymic epithelial cells

• Full Name: activated leukocyte cell adhesion molecule
• Calculated Molecular Weight: 105 kDa
• GenBank Accession Number: BC057809
• Gene Symbol: ALCAM
• Gene ID (NCBI): 214
• RRID: AB_2935249
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: Q13740
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Activated leukocyte cell adhesion molecule (ALCAM, also known as CD166) is a cell adhesion molecule that belongs to the immunoglobulin superfamily. It is involved in cell-cell adhesion through homophilic and heterophilic (to CD6) interactions. ALCAM is widely expressed in a variety of normal tissues and cell types, including activated T cells and monocytes, epithelial cells, fibroblasts, neuronal cells, hepatocytes, and bone marrow mesenchymal stem cells (PMID: 7760007; 25221999). Altered ALCAM expression has been associated with the differentiation state and progression in some neoplasms including melanoma, prostate, colorectal, and breast cancers (PMID: 20461761; 18172759).