ALCAM Polyclonal antibody

ALCAM Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 21972-1-AP

Synonyms

MEMD, CD166

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Validation Data Gallery

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  WB analysis using 21972-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21972-1-AP (ALCAM antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 21972-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 21972-1-AP (ALCAM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 21972-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21972-1-AP (ALCAM antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse liver using 21972-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 21972-1-AP (ALCAM antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 21972-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 21972-1-AP (ALCAM antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 21972-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 21972-1-AP (ALCAM antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 21972-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 21972-1-AP (ALCAM antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 21972-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21972-1-AP (ALCAM Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 21972-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21972-1-AP (ALCAM Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 21972-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 21972-1-AP (ALCAM antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 21972-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 21972-1-AP (ALCAM antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse brain using 21972-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21972-1-AP (ALCAM antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 21972-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21972-1-AP (ALCAM antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A549 cells, human liver tissue, mouse brain tissue, U2OS cells
• Positive IHC detected in: mouse liver tissue, human breast cancer tissue, human colon cancer tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Product Information

21972-1-AP targets ALCAM in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ALCAM fusion protein Ag16892
• Full Name: activated leukocyte cell adhesion molecule
• Calculated Molecular Weight: 583 aa, 65 kDa
• Observed Molecular Weight: 100-110 kDa
• GenBank Accession Number: BC137097
• Gene Symbol: ALCAM
• Gene ID (NCBI): 214
• RRID: AB_2878958
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Activated leukocyte cell adhesion molecule (ALCAM, also known as CD166) is a cell adhesion molecule that belongs to the immunoglobulin superfamily. It is involved in cell-cell adhesion through homophilic and heterophilic (to CD6) interactions. ALCAM is widely expressed in a variety of normal tissues. Altered ALCAM expression has been associated with the differentiation state and progression in some neoplasms including melanoma, prostate, colorectal, and breast cancers (PMID: 20461761; 18172759).

Publications

Species	Application	Title
human	WB	Cancer Discov; Dynamic glycoprotein hyposialylation promotes chemotherapy evasion and metastatic seeding of quiescent circulating tumor cell clusters in breast cancer; Authors - Nurmaa K Dashzeveg
human	IHC	EBioMedicine; Organoid modelling identifies that DACH1 functions as a tumour promoter in colorectal cancer by modulating BMP signalling.; Authors - Xiang Hu
human	WB,IF	Onco Targets Ther; Alpha-1 Antitrypsin Induces Epithelial-to-Mesenchymal Transition, Endothelial-to-Mesenchymal Transition, and Drug Resistance in Lung Cancer Cells.; Authors - Dong-Ming Wu
human	WB,IHC	Cell Death Dis; PMID: 31024010; Authors - Dong-Ming Wu
human,mouse	WB,IHC	Cell Commun Signal; Olanzapine suppresses mPFC activity-norepinephrine releasing to alleviate CLOCK-enhanced cancer stemness under chronic stress; Authors - Jinxin Lu
human	WB,IHC	Cell Metab; Oncogenic fatty acid oxidation senses circadian disruption in sleep-deficiency-enhanced tumorigenesis; Authors - Fei Peng