AIM2 Monoclonal antibody, PBS Only

AIM2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, rabbit

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3E12A8

Cat no : 66902-1-PBS

Synonyms

absent in melanoma 2, AIM2, PYHIN4

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Validation Data Gallery

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  WB analysis using 66902-1-Ig (same clone as 66902-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66902-1-Ig (AIM2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66902-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rabbit spleen using 66902-1-Ig (same clone as 66902-1-PBS)

  rabbit spleen tissue were subjected to SDS PAGE followed by western blot with 66902-1-Ig (AIM2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66902-1-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 66902-1-Ig (same clone as 66902-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66902-1-Ig (AIM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66902-1-PBS in a different storage buffer formulation.

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  WB analysis of human heart using 66902-1-Ig (same clone as 66902-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 66902-1-Ig (AIM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66902-1-PBS in a different storage buffer formulation.

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  IHC staining of rat small intestine using 66902-1-Ig (same clone as 66902-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 66902-1-Ig (AIM2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66902-1-PBS in a different storage buffer formulation.

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  IHC staining of human urothelial carcinoma using 66902-1-Ig (same clone as 66902-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 66902-1-Ig (AIM2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66902-1-PBS in a different storage buffer formulation.

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  IHC staining of human urothelial carcinoma using 66902-1-Ig (same clone as 66902-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 66902-1-Ig (AIM2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66902-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66902-1-PBS targets AIM2 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, rabbit samples.

• Tested Reactivity: Human, mouse, rat, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: AIM2 fusion protein Ag14607
• Full Name: absent in melanoma 2
• Calculated Molecular Weight: 343 aa, 39 kDa
• Observed Molecular Weight: 37-39 and 53 kDa
• GenBank Accession Number: BC010940
• Gene Symbol: AIM2
• Gene ID (NCBI): 9447
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Absent in melanoma 2 (AIM2) is a sensor of cytosolic DNA that is responsible for activation of the inflammasome and host immune responses to DNA viruses and intracellular bacteria [PMID: 22695634]. Upon binding to DNA, it is thought to undergo oligomerization and to associate with PYCARD initiating the recruitment of caspase-1 precusrsor and processing of interleukin-1 beta and interleukin-18. Otherwise, it functions as a tumor suppressor which may act by repressing NF-kappa-B transcriptional activity [PMID:16432157,20566831]. 66902-1-Ig antibody detects the 37-39 and 53 kDa protein in SDS-PAGE similar to the paper published. [PMID: 28809949]