|Positive WB detected in||RAW 264.7 cells, rabbit spleen tissue, human heart tissue, Sp2/0 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Sample-dependent, check data in validation data gallery|
66902-1-Ig targets AIM2 in WB, IF, CoIP, ELISA applications and shows reactivity with Human, mouse, rat, rabbit samples.
|Tested Reactivity||Human, mouse, rat, rabbit|
|Cited Reactivity||human, mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||AIM2 fusion protein Ag14607|
|Full Name||absent in melanoma 2|
|Calculated molecular weight||343 aa, 39 kDa|
|Observed molecular weight||37-39 and 53 kDa|
|GenBank accession number||BC010940|
|Gene ID (NCBI)||9447|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Absent in melanoma 2 (AIM2) is a sensor of cytosolic DNA that is responsible for activation of the inflammasome and host immune responses to DNA viruses and intracellular bacteria [PMID:22695634]. Upon binding to DNA, it is thought to undergo oligomerization and to associate with PYCARD initiating the recruitment of caspase-1 precusrsor and processing of interleukin-1 beta and interleukin-18. Otherwise, it functions as a tumor suppressor which may act by repressing NF-kappa-B transcriptional activity [PMID:16432157,20566831]. 66902-1-Ig antibody detects the 37-39 and 53 kDa protein in SDS-PAGE similar to the paper published. [PMID: 28809949]
STAT3-mediated upregulation of the AIM2 DNA sensor links innate immunity with cell migration to promote epithelial tumourigenesis.
Establishment of tumor inflammasome clusters with distinct immunogenomic landscape aids immunotherapy
PKIB involved in the metastasis and survival of osteosarcoma
Cell Commun Signal
Increased susceptibility of irradiated mice to Aspergillus fumigatus infection via NLRP3/GSDMD pathway in pulmonary bronchial epithelia.
Sci Total Environ
Cadmium exposure induces pyroptosis in testicular tissue by increasing oxidative stress and activating the AIM2 inflammasome pathway.
Prohibitin 1 regulates mtDNA release and downstream inflammatory responses