AIM2 Monoclonal antibody
AIM2 Monoclonal Antibody for WB,ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rat, rabbit and More (1)
Applications
WB, IF, CoIP, ELISA
Conjugate
Unconjugated
CloneNo.
3E12A8
Cat no : 66902-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | RAW 264.7 cells, rabbit spleen tissue, human heart tissue, Sp2/0 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 1 publications below |
WB | See 5 publications below |
IF | See 1 publications below |
CoIP | See 1 publications below |
Product Information
66902-1-Ig targets AIM2 in WB, IF, CoIP, ELISA applications and shows reactivity with Human, mouse, rat, rabbit samples.
Tested Reactivity | Human, mouse, rat, rabbit |
Cited Reactivity | human, mouse |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | AIM2 fusion protein Ag14607 |
Full Name | absent in melanoma 2 |
Calculated molecular weight | 343 aa, 39 kDa |
Observed molecular weight | 37-39 and 53 kDa |
GenBank accession number | BC010940 |
Gene symbol | AIM2 |
Gene ID (NCBI) | 9447 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Absent in melanoma 2 (AIM2) is a sensor of cytosolic DNA that is responsible for activation of the inflammasome and host immune responses to DNA viruses and intracellular bacteria [PMID:22695634]. Upon binding to DNA, it is thought to undergo oligomerization and to associate with PYCARD initiating the recruitment of caspase-1 precusrsor and processing of interleukin-1 beta and interleukin-18. Otherwise, it functions as a tumor suppressor which may act by repressing NF-kappa-B transcriptional activity [PMID:16432157,20566831]. 66902-1-Ig antibody detects the 37-39 and 53 kDa protein in SDS-PAGE similar to the paper published. [PMID: 28809949]
Protocols
Product Specific Protocols | |
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WB protocol for AIM2 antibody 66902-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Gut STAT3-mediated upregulation of the AIM2 DNA sensor links innate immunity with cell migration to promote epithelial tumourigenesis. | ||
Theranostics Establishment of tumor inflammasome clusters with distinct immunogenomic landscape aids immunotherapy | ||
Cell Commun Signal Increased susceptibility of irradiated mice to Aspergillus fumigatus infection via NLRP3/GSDMD pathway in pulmonary bronchial epithelia. | ||
Sci Total Environ Cadmium exposure induces pyroptosis in testicular tissue by increasing oxidative stress and activating the AIM2 inflammasome pathway. | ||
EMBO J Prohibitin 1 regulates mtDNA release and downstream inflammatory responses
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