Tested Applications
| Positive WB detected in | mouse brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human hepatocirrhosis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20101-1-AP targets ACTR10 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13612 Product name: Recombinant human ACTR10 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-417 aa of BC011997 Sequence: MPLYEGLGSGGEKTAVVIDLGEAFTKCGFAGETGPRCIIPSVIKRAGMPKPVRVVQYNINTEELYSYLKEFIHILYFRHLLVNPRDRRVVIIESVLCPSHFRETLTRVLFKYFEVPSVLLAPSHLMALLTLGINSAMVLDCGYRESLVLPIYEGIPVLNCWGALPLGGKALHKELETQLLEQCTVDTSVAKEQSLPSVMGSVPEGVLEDIKARTCFVSDLKRGLKIQAAKFNIDGNNERPSPPPNVDYPLDGEKILHILGSIRDSVVEILFEQDNEEQSVATLILDSLIQCPIDTRKQLAENLVVIGGTSMLPGFLHRLLAEIRYLVEKPKYKKALGTKTFRIHTPPAKANCVAWLGGAIFGALQDILGSRSVSKEYYNQTGRIPDWCSLNNPPLEMMFDVGKTQPPLMKRAFSTEK Predict reactive species |
| Full Name | actin-related protein 10 homolog (S. cerevisiae) |
| Calculated Molecular Weight | 417 aa, 46 kDa |
| Observed Molecular Weight | 46 kDa |
| GenBank Accession Number | BC011997 |
| Gene Symbol | ACTR10 |
| Gene ID (NCBI) | 55860 |
| RRID | AB_10640424 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NZ32 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ACTR10 antibody 20101-1-AP | Download protocol |
| IHC protocol for ACTR10 antibody 20101-1-AP | Download protocol |
| IP protocol for ACTR10 antibody 20101-1-AP | Download protocol |
| WB protocol for ACTR10 antibody 20101-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ACTR10 Polyclonal antibody (Cat# 20101-1-AP) has 4 total citations. These appear in PNAS, Current Biology, Neurobiology of Aging, and Molecular Biology of the Cell. The research fields span neurobiology (myelination, dystrophic neurite formation), cell biology (spindle orientation, actin regulation), and intracellular transport (cargo targeting by the dynactin complex). All cited applications are Western blot, with species including human, mouse, rat, and monkey.
| Species | Application | Title |
|---|---|---|
Proc Natl Acad Sci U S A Dynein/dynactin is necessary for anterograde transport of Mbp mRNA in oligodendrocytes and for myelination in vivo. | ||
Curr Biol An RNAi Screen in a Novel Model of Oriented Divisions Identifies the Actin-Capping Protein Z β as an Essential Regulator of Spindle Orientation. | ||
Neurobiol Aging Dynactin 6 deficiency enhances aging-associated dystrophic neurite formation in mouse brains. | ||
Reviews
What customers say
The product has one verified review with a 5-star rating. The reviewer used it at a 1:1000 dilution on N2A, 293T, and ReN cells for Western Blot and Immunohistochemistry, reporting that it works great in their lab. Overall, the feedback is positive, confirming reliable performance across multiple cell types and applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Zakia (Verified Customer) (05-22-2024) | we are using this for our lab, Works great,
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