Gamma Actin Polyclonal antibody

Gamma Actin Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 11227-1-AP

Synonyms

ACTG1, Actin, cytoplasmic 2, N-terminally processed, Actin, cytoplasmic 2, actin, actg 1

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Validation Data Gallery

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  WB analysis using 11227-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11227-1-AP (gamma Actin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin cancer using 11227-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 11227-1-AP (Gamma Actin antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 11227-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 11227-1-AP (Gamma Actin antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 11227-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11227-1-AP (Gamma Actin antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse lung tissue, rat lung tissue
• Positive IHC detected in: human colon tissue, human skin cancer tissue, human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochemistry (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IF: See 1 publications below

Product Information

11227-1-AP targets Gamma Actin in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Gamma Actin fusion protein Ag1728
• Full Name: actin, gamma 1
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 42-45 kDa
• GenBank Accession Number: BC009848
• Gene Symbol: Gamma Actin
• Gene ID (NCBI): 71
• RRID: AB_2223507
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	iScience; A defective mechanosensing pathway affects fibroblast-to-myofibroblast transition in the old male mouse heart; Authors - Aude Angelini
mouse	WB	Front Mol Neurosci; F-Actin Dysplasia Involved in Organ of Corti Deformity in Gjb2 Knockdown Mouse Model.; Authors - Xiao-Zhou Liu
human	WB	Front Neurosci; Detergent-insoluble PFN1 inoculation expedites disease onset and progression in PFN1 transgenic rats; Authors - Shiquan Cui
mouse	WB	FEBS J; Icariin ameliorates glycolytic dysfunction in Alzheimer's disease models by activating the Wnt/β-catenin signaling pathway; Authors - Ju Liu
human	WB	Ultrasound Q; Ultrasound Microbubble-Stimulated miR-145-5p Inhibits Malignant Behaviors of Breast Cancer Cells by Targeting ACTG1; Authors - Liang Ren
human	WB,IF	Cells; Interaction of Receptor-Binding Domain of the SARS-CoV-2 Omicron Variant with hACE2 and Actin; Authors - Ai Fujimoto