Alpha Actin Polyclonal antibody

Alpha Actin Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11032-1-AP

Synonyms

ACTC, ACTC1, ACTC1 cardiac muscle 1, Actin, alpha cardiac muscle 1, actin, alpha, cardiac muscle 1, Alpha actin, Alpha cardiac actin, CMD1R, CMH11



Tested Applications

Positive WB detected inmouse heart tissue, mouse skeletal muscle tissue, mouse thymus tissue
Positive IP detected inmouse heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

11032-1-AP targets Alpha Actin in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Alpha Actin fusion protein Ag1471
Full Name actin, alpha, cardiac muscle 1
Calculated molecular weight 377 aa, 42 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC009978
Gene symbol ACTC1
Gene ID (NCBI) 70
RRIDAB_2242376
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Alpha Actin antibody 11032-1-APDownload protocol
IP protocol for Alpha Actin antibody 11032-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

FASEB J

Zbtb20 deficiency causes cardiac contractile dysfunction in mice.

Authors - An-Jing Ren
humanWB

Int J Biol Sci

MnSOD Lysine 68 acetylation leads to cisplatin and doxorubicin resistance due to aberrant mitochondrial metabolism.

Authors - Yucheng Gao
humanIHC

Stem Cells Dev

A model of early human embryonic stem cell differentiation reveals inter- and intracellular changes on transition to squamous epithelium.

Authors - Galat Vasiliy V
humanWB

Onco Targets Ther

Clinical Significance and Oncogenic Activity of GRWD1 Overexpression in the Development of Colon Carcinoma.

Authors - Xin Zhou