ACSL5 Polyclonal antibody, PBS Only

ACSL5 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 15708-1-PBS

Synonyms

LACS 5, FACL5, EC:6.2.1.3, EC:6.2.1.15, Arachidonate--CoA ligase

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Validation Data Gallery

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  WB analysis using 15708-1-AP (same clone as 15708-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15708-1-AP (ACSL5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15708-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 15708-1-AP (same clone as 15708-1-PBS)

  IP result of anti-ACSL5 (IP:15708-1-AP, 4ug; Detection:15708-1-AP 1:600) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 15708-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 15708-1-AP (same clone as 15708-1-PBS)

  Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 15708-1-AP (ACSL5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15708-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 15708-1-AP (same clone as 15708-1-PBS)

  Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 15708-1-AP (ACSL5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15708-1-PBS in a different storage buffer formulation.

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Product Information

15708-1-PBS targets ACSL5 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ACSL5 fusion protein Ag8258
• Full Name: acyl-CoA synthetase long-chain family member 5
• Calculated Molecular Weight: 76 kDa
• Observed Molecular Weight: 66-70 kDa
• GenBank Accession Number: BC007985
• Gene Symbol: ACSL5
• Gene ID (NCBI): 51703
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Acyl-CoA synthetase 5, a member of the ACSL gene family that catalyzes the activation of long-chain fatty acids for lipid biosynthesis, is the only ACSL isoform that is both, located on mitochondria and functionally involved in enterocyte apoptosis.ACSL5 could play a role in promoting fatty acid-induced lipoapoptosis in hepatocytes as important mechanism in fatty liver-related disorders(PMID:20470896). ACSL5 is most abundant in liver, brown adipose tissue, and intestine and is located on both the mitochondrial membrane and endoplasmic reticulum(PMID:20798351). It has 3 isoforms produced by alternative splicing.