ACADS Polyclonal antibody

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  WB analysis using 16623-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16623-1-AP (ACADS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of COS-7 using 16623-1-AP

  COS-7 cells were subjected to SDS PAGE followed by western blot with 16623-1-AP (ACADS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 16623-1-AP

  IP result of anti-ACADS (IP:16623-1-AP, 4ug; Detection:16623-1-AP 1:500) with HEK-293 cells lysate 3000ug.

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  IHC staining of human kidney using 16623-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16623-1-AP (ACADS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 16623-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16623-1-AP (ACADS antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 16623-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16623-1-AP (ACADS antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 16623-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16623-1-AP (ACADS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 16623-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16623-1-AP (ACADS antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human heart using 16623-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16623-1-AP (ACADS antibody) at dilution of 1:100 (under 40x lens).

• Featured Product
• KD/KO Validated

ACADS Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 16623-1-AP

Publications(21)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

WB, IHC, IF, IP, ELISA

Synonyms

ACAD3, ACADS, Butyryl CoA dehydrogenase, SCAD

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Tested Applications

• Positive WB detected in: mouse kidney tissue, COS-7 cells, mouse liver tissue, rat kidney tissue, rat liver tissue
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human kidney tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 18 publications below
• IF: See 2 publications below
• IP: See 3 publications below

Product Information

16623-1-AP targets ACADS in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

• Tested Reactivity: human, mouse, rat, monkey
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9922

  Product name: Recombinant human ACADS protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-412 aa of BC025963

  Sequence: MAAALLARASGPARRALCPRAWRQLHTIYQSVELPETHQMLLQTCRDFAEKELFPIAAQVDKEHLFPAAQVKKMGGLGLLAMDVPEELGGAGLDYLAYAIAMEEISRGCASTGVIMSVNNSLYLGPILKFGSKEQKQAWVTPFTSGDKIGCFALSEPGNGSDAGAASTTARAEGDSWVLNGTKAWITNAWEASAAVVFASTDRALQNKSISAFLVPMPTPGLTLGKKEDKLGIRGSSTANLIFEDCRIPKDSILGEPGMGFKIAMQTLDMGRIGIASQALGIAQTALDCAVNYAENRMAFGAPLTKLQVIQFKLADMALALESARLLTWRAAMLKDNKKPFIKEAAMAKLAASEAATAISHQAIQILGGMGYVTEMPAERHYRDARITEIYEGTSEIQRLVIAGHLLRSYRS

• Full Name: acyl-Coenzyme A dehydrogenase, C-2 to C-3 short chain
• Calculated Molecular Weight: 412 aa, 44 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC025963
• Gene Symbol: ACADS
• Gene ID (NCBI): 35
• RRID: AB_10666165
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P16219
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACADS is also named as SCAD(Short-chain specific acyl-CoA dehydrogenase, mitochondrial) and belongs to the acyl-CoA dehydrogenase family. It is a tetrameric mitochondrial flavoprotein. It is synthesized as a 44 kDa precursor, transported into mitochondria, and proteolytically processed to its 41 kDa mature form. Acyl-CoA de- hydrogenases (ACADs) are a family of flavoproteins that are involved in theβ-oxidation of the fatty acyl-CoA derivatives.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Cell Res; Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation; Authors - Yunzi Mao
mouse	WB	Cell Metab; Targeting Erbin-mitochondria axis in platelets/megakaryocytes promotes B cell-mediated antitumor immunity; Authors - Zilong Zhang
human	WB	EMBO Rep; Lin28 enhances de novo fatty acid synthesis to promote cancer progression via SREBP-1.; Authors - Yang Zhang
human	WB	Cell Rep; HIF-1-Mediated Suppression of Acyl-CoA Dehydrogenases and Fatty Acid Oxidation Is Critical for Cancer Progression.; Authors - De Huang
human	WB	Aging (Albany NY); ACADS acts as a potential methylation biomarker associated with the proliferation and metastasis of hepatocellular carcinomas.; Authors - Diyu Chen ; KD Validated
mouse, rat	WB	Arch Toxicol; Protective effects of dioscin against alcohol-induced liver injury.; Authors - Xu Tingting T