ABLIM1 Polyclonal antibody

• Featured Product
• KD/KO Validated

ABLIM1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

Publications

6

Reviews (1)

Cat no : 15129-1-AP

Synonyms

Actin-binding LIM protein family member 1, Actin-binding LIM protein 1, Actin-binding double zinc finger protein, actin binding LIM protein 1, abLIM-1

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Validation Data Gallery

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  WB analysis of HeLa using 15129-1-AP

  WB result of ABLIM1 antibody (15129-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ABLIM1 transfected HeLa cells.

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  WB analysis of NCI-H1299 using 15129-1-AP

  NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 15129-1-AP (ABLIM1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 15129-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15129-1-AP (ABLIM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15129-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15129-1-AP (ABLIM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 15129-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15129-1-AP (ABLIM1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 15129-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15129-1-AP (ABLIM1 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of mouse skeletal muscle using 15129-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using 15129-1-AP (ABLIM1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse skeletal muscle using 15129-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using 15129-1-AP (ABLIM1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HEK-293 using 15129-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 15129-1-AP (ABLIM1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, NCI-H1299 cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse skeletal muscle tissue
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 5 publications below
• IF: See 2 publications below

Product Information

15129-1-AP targets ABLIM1 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ABLIM1 fusion protein Ag7258
• Full Name: actin binding LIM protein 1
• Calculated Molecular Weight: 88 kDa
• Observed Molecular Weight: 48-52 kDa, 105-110 kDa
• GenBank Accession Number: BC002448
• Gene Symbol: ABLIM1
• Gene ID (NCBI): 3983
• RRID: AB_2257767
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ABLIM1 (actin-binding LIM protein 1) is a member of the LIM-domain protein family, mediating interactions between actin filaments and cytoplasmic targets. ABLIM1 has been reported as a cell cortex protein specifically in non-erythrocytes, and is critical for the formation of dense interwoven cortical actin meshwork. Several isoforms of ABLIM1 exist due to the alternative splicing. This antibody recognized 110 kDa and 48 kDa forms of ABLIM1. The phosphorylation or binding with SUMO2 may result in higher MW (predicted MW 88 kDa).

Publications

Species	Application	Title
human	WB	Nat Cell Biol; miR-129-3p controls cilia assembly by regulating CP110 and actin dynamics.; Authors - Cao Jingli J ; KD Validated
mouse	WB	J Cell Physiol; Actin binding LIM 1 (abLIM1) negatively controls osteoclastogenesis by regulating cell migration and fusion.; Authors - Haruna Narahara ; KD Validated
human	WB	Int J Biol Sci; Rictor promotes cell migration and actin polymerization through regulating ABLIM1 phosphorylation in Hepatocellular Carcinoma.; Authors - Xin Dong ; KD Validated
	WB	Mol Biol Cell; Spatial proximity of proteins surrounding zyxin under force-bearing conditions.; Authors - Joleen S Cheah
human	IF	medRxiv; Antigenic responses are hallmarks of fibrotic interstitial lung diseases independent of underlying etiologies; Authors - Young Me Yoon
mouse	WB,IF	Circ Res; GSK-3β Localizes to the Cardiac Z-Disc to Maintain Length Dependent Activation; Authors - Marisa J Stachowski-Doll

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Joleen (Verified Customer) (05-29-2019)

ABLIM should be around 87kD, but the 2 prominent bands do not come near that molecular weight. The top band is just above the 100kD molecular weight marker and the lower band is below the 75kD molecular weight marker. (BioRad Precision Plus Protein Standard used).

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: MDCK GII

ABLIM1 Antibody Western Blot, validation (1:1000 dilution) in MDCK GII (Cat no:15129-1-AP)