ABL2 Polyclonal antibody

• Featured Product
• KD/KO Validated

ABL2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 17693-1-AP

Synonyms

Abelson related gene protein, ABL2, ABLL, ARG, Tyrosine protein kinase ABL2, Tyrosine protein kinase ARG

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Validation Data Gallery

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  WB analysis using 17693-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17693-1-AP (ABL2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 17693-1-AP

  IP result of anti-ABL2 (IP:17693-1-AP, 4ug; Detection:17693-1-AP 1:500) with HeLa cells lysate 1200ug.

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  IHC staining of human gliomas using 17693-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 17693-1-AP (ABL2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 17693-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 17693-1-AP (ABL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Product Information

17693-1-AP targets ABL2 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ABL2 fusion protein Ag11860
• Full Name: v-abl Abelson murine leukemia viral oncogene homolog 2 (arg, Abelson-related gene)
• Calculated Molecular Weight: 1167 aa, 127 kDa
• Observed Molecular Weight: 128-140 kDa
• GenBank Accession Number: BC065912
• Gene Symbol: ABL2
• Gene ID (NCBI): 27
• RRID: AB_2289025
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ABL2(Abelson tyrosine-protein kinase 2) is also named as ABLL, ARG and belongs to the protein kinase superfamily. It is a membrane-anchored nonreceptor tyrosine kinase that is a novel mediator of SEMA3F-induced RhoA inactivation and collapsing activity and it has a role in cytoskeletal rearrangements by its C-terminal F-actin- and microtubule-binding sequences. ABL2 can be polyubiquitinated and the polyubiquitination of ABL2 leads to degradation. This protein has 9 isoforms produced by alternative splicing with the molecular weight from 114 kDa to 128 kDa. The antibody is specific to the others except the 60 kDa isoform of ABL2.

Publications

Species	Application	Title
	IF	Bioact Mater; High proportion strontium-doped micro-arc oxidation coatings enhance early osseointegration of titanium in osteoporosis by anti-oxidative stress pathway.; Authors - Xinkun Shen
	WB	Theranostics; Non-invasive photobiomodulation treatment in an Alzheimer Disease-like transgenic rat model.; Authors - Luodan Yang
human	WB	Elife; Abelson tyrosine-protein kinase 2 regulates myoblast proliferation and controls muscle fiber length.; Authors - Jennifer K Lee ; KO Validated
human	WB,CoIP,IHC	Oncogene; Phosphorylation of MICAL2 by ARG promotes head and neck cancer tumorigenesis by regulating skeletal rearrangement; Authors - Ze Zhang
human	WB	Genomics; Bone marrow mesenchymal stem cells-derived exosomal microRNA-19b-1-5p reduces proliferation and raises apoptosis of bladder cancer cells via targeting ABL2.; Authors - Dewang Fu
human	WB	Biochim Biophys Acta; Abl2 kinase phosphorylates Bi-organellar regulator MNRR1 in mitochondria, stimulating respiration.; Authors - Siddhesh Aras